NtMYB17过表达对烟草腺毛发育的影响

Effect of NtMYB17 Overexpression on Development of Glandular Trichomes in Tobacco

作为植物预防生物和非生物胁迫的第一道防线,以及次生代谢物的分泌和合成场所,烟草腺毛的分子调控研究对烟草抵抗逆境胁迫和品质育种具有重要意义。为明确烟草MYB转录因子NtMYB17在调控腺毛发育过程中的生物学功能,在普通烟草中克隆了NtMYB17基因,并对其保守结构域和基因结构进行分析,利用qRT-PCR技术分析了NtMYB17的表达模式,进一步结合亚细胞定位试验和过表达试验验证了基因的功能。结果表明:NtMYB17基因全长939 bp,共编码312个氨基酸,具有典型的MYB结构域,亚细胞定位于细胞核中;qRT-PCR结果显示,NtMYB17在腺毛中的表达量是去腺毛叶片中表达量的8倍,且在花蕾和幼叶中的表达量较高,在根中的表达量最低;在过表达NtMYB17基因的烟草植株中分枝型腺毛数量增加。该研究表明NtMYB17基因对烟草腺毛的形态发育具有调控作用。

As the front line of biological and abiotic defense,and the site of secretion and synthesis of secondary metabolites,regulation of tobacco glandular hair is of great importance for tobacco stress resistance and quality breeding.To explore the function of MYB transcript factors in tobacco glandular trichomes development,one MYB transcription factor NtMYB17 gene were cloned from common tobacco in this study.The analysis involved examining the conserved domain,assessing tissue-specific gene expression via qRT-PCR,and conducting a subcellular localization to further scrutinize the expression pattern of the gene.Results showed that NtMYB17 had a total length of 939 bp,encoding 312 amino acids,a typical MYB domain and was localized in the nucleus at the subcellular level.qRT-PCR revealed that the expression of NtMYB17 in glandular trichomes was eight times higher than that in trichome-free leaves.The expression level was higher in flower buds and young leaves,and it was lowest in roots.Following the overexpression of NtMYB17 in tobacco,an increase was observed in the number of branch-type glandular trichomes.This study indicated that NtMYB17 played a regulatory role in the morphology of tobacco glandular trichomes.