基于新型固定液制备小鼠AMD模型和人胚胎眼球高质量视网膜石蜡切片

Preparation of high quality retinal paraffin sections of mouse AMDmodel and human embryonic eye based on a novel fixative

将C57/B6J小鼠分别固定于传统固定液(4%甲醛液,下同)与新型固定液中,进行石蜡切片的常规制片程序,并将新型固定液在年龄相关性黄斑变性(AMD)模型小鼠及人胚胎眼球HE染色石蜡切片中进行效果验证。用新型固定液处理的眼球石蜡切片质量优于传统固定液,且成功模拟了AMD模型小鼠视网膜各层不同时间段的变化。新型固定液在制备高质量视网膜石蜡切片方面是可行的,为后续视网膜石蜡切片的有关研究奠定了基础。

C57/B6J mice were fixed in traditional fixative(4%formaldehyde solution,the same below)and novel fixative respectively,and the routine procedure of paraffin section preparation was performed,as well as the novel fixative was validated in HE-stained paraffin sections of the eyes of mice and human embryos in the age-related macular degeneration(AMD)model.Compared with the traditional fixative,the quality of the paraffin sections treated with the novel fixative was better than that of the traditional fixative,and successfully simulated the changes of the retinal layers of AMD mice at different time periods.The novel fixative is feasible for the preparation of high-quality retinal paraffin sections,which lays the foundation for subsequent studies on retinal paraffin sections.