基于16sRNA测序分析亚油酸对小鼠肠道菌群的影响

The effects of linoleic acid on intestinal flora in mice were analyzed based on 16sRNA sequencing

目的探究亚油酸(LA)对小鼠肠道菌群多样性和结构的影响。方法将7周12只SPF级雄性C57BL/6J小鼠随机分为对照组(CTRL组)和亚油酸组(LA组)。在补充LA饮食前1 d,对LA组撤去正常粮食,禁食一晚,以便LA组小鼠更易接受LA饮食,且于实验记录当天给予LA,随时保持饲料更新,保证小鼠能自由饮食到造模结束。于造模12周后收集小鼠粪便,每2只小鼠粪便进行混合采样,后续进行16sRNA高通量测序分析肠道菌群结构、Alpha多样性、Beta多样性等分析。结果通过16sRNA高通量测序发现,LA干预后肠道菌群的丰富度及多样性受损;主成分分析结果表明,CTRL组与LA组菌群组成相差较多。在门水平中,LA组放线菌门相对丰富度增加(P<0.01);在属水平中,LA组杜氏乳杆菌属相对丰富度下降(P<0.05),双歧杆菌、粪杆菌属和丹毒丝菌属相对丰富度升高(均P<0.01)。结论LA干预后降低小鼠肠道菌群的丰富度与多样性,且调整肠道菌群的结构。LA干预后的肠道菌群中有益菌及致病菌存在差异,为LA的生物活性化合物的疗法和以肠道微生物为靶点的治疗调整提供一定的依据。

Objective To investigate the effects of linoleic acid(LA)on the diversity and structure of intestinal flora in mice.Methods Twelve SPF grade male C57BL/6J mice at 7 weeks were randomly divided into control group(CTRL group)and linoleic acid group(LA group).One day before the linoleic acid diet was supplemented,the normal food was removed from the LA group and the mice in the LA group were fasted for one night,so that the LA diet was more acceptable to the mice in the LA group,and LA was given on the day of the experiment recording,and the feed was updated at any time to ensure that the mice could eat freely until the end of modeling.After 12 weeks of modeling,mouse feces were collected,and mixed samples were collected for every two mice feces,and then 16sRNA high-throughput sequencing was performed to analyze intestinal flora structure,Alpha and Beta diversity.Results 16sRNA high-throughput sequencing showed that LA intervention damaged the richness and diversity of intestinal flora.The results of principal component analysis showed that the composition of flora in CTRL group was different from that in LA group.At gate level,the relative abundance of Actinobacteria in LA group increased(P<0.01).At the genus level,the relative abundance of L.Duchennei in the LA group decreased(P<0.05),but the relative abundance of Bifidobacterium,Faecalibaculum and Erysipelotrichaceae in the LA group increased(all P<0.01).Conclusion LA intervention could reduce the richness and diversity of intestinal flora in mice,and adjust the structure of intestinal flora.There were significant differences between beneficial bacteria and pathogenic bacteria in intestinal flora after LA intervention,which provided certain basis for the treatment of bioactive compounds of linoleic acid and the therapeutic adjustment of intestinal microorganisms as targets.