金银花中多菌灵和吡虫啉残留基体标准样品的研制

Development of matrix standard sample of carbendazim and imidacloprid residue in Lonicera japonica

[目的]建立金银花中多菌灵、吡虫啉残留基体标准样品的研制方法。[方法]将空白的干金银花进行喷药、阴干、粉碎、过筛、混匀、分装、水分检测。采用高效液相色谱法测定,外标法定量,经均匀性和稳定性检验后,3种分析方法对其联合定值,并对不确定度进行评估。[结果]通过定值和不确定度评估得到金银花中多菌灵、吡虫啉残留基体标准样品的特性值,多菌灵特性值为(1.043±0.056)mg/kg,吡虫啉特性值为(0.995±0.042)mg/kg,该基体标准样品均匀性良好,可在-18℃条件下稳定保存。[结论]通过该方法制备的基体标准样品具有良好的均匀性与稳定性,能够应用于检测过程中质量控制、分析仪器校准、检测方法确认评估等。

[Aims]The aim of this study is to establish a method for the development of matrix standard samples of carbendazim and imidacloprid residues in Lonicera japonica.[Methods]The blank dried L.japonica was sprayed,dried in the shade,crushed,sifted,mixed,packed and tested for moisture.It was determined by high performance liquid chromatography and quantified by external standard method.After uniformity and stability tests,three analysis methods were combined to determine the value,and the uncertainty was evaluated.[Results]The characteristic values of carbendazim and imidacloprid residue matrix standard samples in L.japonica were obtained by fixed value and uncertainty evaluation.The characteristic value of carbendazim was(1.043±0.056)mg/kg,while that of imidacloprid was(0.995±0.042)mg/kg.The matrix standard sample had g0od uniformity and could be stored stably at-18°C.[Conclusions]The matrix standard sample prepared by this method has good uniformity and stability,and can be applied for quality control,analytical instrument calibration,detection method confirmation and evaluation in the detection process.