45%二甲戊灵微胶囊悬浮剂对燕麦田土壤微生物群落的影响

Effect of a 45% pendimethalin capsule suspension on the soil microbial community

【目的】研究45%二甲戊灵微胶囊悬浮剂对燕麦(Avena sativa)田土壤微生物群落的影响,为45%二甲戊灵微胶囊悬浮剂应用于燕麦田提供依据。【方法】用扩增子测序评价45%二甲戊灵微胶囊悬浮剂对土壤微生物群落结构的影响。【结果】燕麦田表层土壤微生物多样性评价结果显示:施用二甲戊灵30 d后土壤微生物观察到的物种(Observed species)、Chao1、Shannon和Simpson多样性指数与对照之间均无显著性差异。用多重响应排列程序、相似性分析和置换多元方差分析3种方法对测序结果进行主成分分析,结果显示:二甲戊灵对土壤微生物多样性无显著影响。在内蒙古农业大学农场试验点,二甲戊灵施用后节杆菌属(Arthrobacter)内微生物种群丰度明显降低;在乌兰察布市农林科学研究所试验田,二甲戊灵施用后真菌出壳多孢菌属(Stagonosporopsis)内微生物种群丰度显著降低,镰刀菌属(Fusarium)和短梗蠕孢属(Trichocladium)内微生物种群丰度显著升高。【结论】节杆菌属(Arthrobacter)、出壳多孢菌(Stagonosporopsis)、镰刀菌属(Fusarium)和短梗蠕孢属(Trichocladium)内微生物对二甲戊灵相对敏感,但45%二甲戊灵微胶囊悬浮剂不影响土壤微生物多样性指数。

【Objective】 To evaluate the safety of 45% pendimethalin capsule suspension on soil microbial diversity.【Method】 The amplicon sequencing of the soil microbiology has been carried out.【Result】 The sequencing results could reflect the microbial diversity of the soil samples.The 45% pendimethalin capsule suspension did not have a significant effect on soil microbial diversity based on the stability of the multiple diversity index,observed species,Chao1,Shannon and Simpson(P>0.05).Principal Component Analysis(PCA) also showed that the herbicide did not affect the soil microbial community.The abundance of bacteria in the genus Arthrobacter decreased significantly at the Ulanchap Institute of Agricultural and Forestry Sciences site.At the Inner Mongolia Agricultural University site,the abundance of fungi in the genus Stagonosporopsis decreased significantly,while the abundance of fungi in the genus Fusarium and Trichocladium increased.【Conclusion】 45% pendimethalin capsule suspension had no significant effect on the soil microbial community and diversity of the oat field.