两种矿物源农药与化学农药混配对百合灰霉病菌抑菌增效作用评价

Evaluation of the synergistic effect of two mineral and chemical fungicides mixed on Botrytis cinerea

【目的】为明确矿物源农药与化学农药混配对百合灰霉病的防治效果。【方法】采用菌落生长速率法、黄瓜子叶法及喷雾法测定了氧化亚铜、石硫合剂分别与3种化学农药混配对百合灰霉菌的抑菌增效作用、离体防效、室内盆栽防效及持效期和对百合的安全性。【结果】混配剂86.2%氧化亚铜WP∶70%噁霉灵SP(9∶1)及混配剂29%石硫合剂AS∶75%百菌清WP(9∶1)抑菌活性最强,EC50分别为99.90μg/mL、105.58μg/mL,CTC分别为130.65、122.28,表现为增效作用,其中混配剂86.2%氧化亚铜WP∶70%噁霉灵SP以9∶1混配时对百合灰霉病的防效最好,保护作用防效为87.01%,治疗作用防效为81.81%,室内盆栽防效测定表明第7天防效最大,为76.18%,持效期为7 d。【结论】试验所选药剂对兰州百合安全,86.2%氧化亚铜WP∶70%噁霉灵SP(9∶1)混剂防效高于单剂防效,而29%石硫合剂AS∶75%百菌清WP(9∶1)混剂的防效则低于其单剂防效。

【Objective】 To determine the control effect of the mixture of mineral and chemical pesticides on the lily Botrytis cinerea.【Method】 The inhibition synergistic effect,in vitro control effect,indoor pot control effect and duration of the mixture of cuprous oxide,lime-sulphur mixture and three chemical fungicides on B.cinerea on lily and their safety to lily were determined by using the colony growth rate method,the cucumber cotyledon method and the spray method.【Result】 The mixtures of 86.2% cuprous oxide WP:70% oxazole SP(9∶1) and 29% sulphur mixture AS∶75% chlorothalonil WP(9∶1) had the strongest toxicity,EC50 were 99.90 μg/mL and 105.58 μg/mL,and CTC were 130.65 and 122.28,respectively,showing a synergistic effect.Among them,the mixture of 86.2% cuprous oxide WP∶70% hymexazol SP(9∶1) had the best control effect on gray mold of lily,with the protection effect and treatment effect were 87.01% and 81.81%,the results of pot experiment in room showed that the largest control effect was 76.18% on the seventh day,with a persistent effect period of 7 d.【Conclusion】 The fungicides selected in the trial was safe for Lanzhou lily in Lanzhou.The control effect of the mixture was higher than that of the single agents of 86.2% cuprous oxide WP∶70% hymexazol SP(9∶1),while the control effect of the mixture of 29% limesulphur mixture AS:75% chlorothalonil WP(9∶1) was lower than that of the single agents.