RECK、MMP-2、bcl-2蛋白在牙龈瘤患儿牙龈组织中的表达分析

Expression of RECK,MMP-2 and bcl-2 Protein in Gingival Tissue of Children with Gingival Tumor

目的 探讨RECK、基质金属蛋白酶-2(MMP-2)、B淋巴细胞瘤-2基因(bcl-2)蛋白在牙龈瘤患儿牙龈组织中的表达情况。方法 选取80例牙龈瘤患儿作为研究对象,所有患儿均行手术治疗,采集肿瘤组织及肿瘤旁健康牙龈组织,采用免疫组化SP法检测样本内RECK、MMP-2、bcl-2蛋白表达情况,比较肿瘤组织及健康牙龈组织中上述表达情况;并随访1年,分析RECK、MMP-2、bcl-2蛋白表达与其复发的关系。结果 肿瘤组织中RECK阳性率为36.25%,低于健康牙龈组织的77.50%,MMP-2阳性率、bcl-2蛋白阳性率分别为73.75%、67.50%,高于对照组的18.75%、22.50%,差异有统计学意义(P<0.05);80例患儿术后随访1年,共出现33例复发,复发率为41.25%(33/80);复发组RECK阳性率为15.15%,低于未复发组的51.06%,MMP-2阳性率、bcl-2蛋白阳性率为90.91%、93.94%,高于未复发组的61.70%、48.94%,差异有统计学意义(P<0.05)。结论 RECK、MMP-2、bcl-2蛋白在牙龈瘤患儿牙龈组织内存在不同表达,RECK阳性表达率低,MMP-2、bcl-2蛋白表达偏高,其表达与复发存在密切关系,可为完善早期治疗工作提供一定指导。

Objective To investigate the expression of RECK,matrix metalloproteinase-2(MMP-2),and B-lymphomatoma-2 gene(bcl-2)proteins in the gingival tissue of children with gingival tumors.Methods 80 children with gingival tumors were selected as the study subjects,and all patients underwent surgical treatment.Tumor tissue and healthy gingival tissue adjacent to the tumor were collected.Immunohistochemical SP method was used to detect the expression of RECK,MMP-2,and bcl-2 proteins in the samples,and the expression of the above proteins in tumor tissue and healthy gingival tissue were compared;Followed up for 1 year to analyze the relationship between RECK,MMP-2,and bcl-2 protein expression and recurrence.Results The positive rate of RECK in tumor tissue was 36.25%,lower than 77.50%in healthy gingival tissue.The positive rates of MMP-2 and bcl-2 protein were 73.75%and 67.50%,respectively,higher than the control group's 18.75%and 22.50%,with statistical significance(P<0.05);80 patients were followed up for 1 year after surgery,and a total of 33 cases experienced recurrence,with a recurrence rate of 41.25%(33/80);The positive rate of RECK in the recurrent group was 15.15%,lower than 51.06%in the non recurrent group.The positive rates of MMP-2 and bcl-2 protein were 90.91%and 93.94%,higher than 61.70%and 48.94%in the non recurrent group,with statistical significance(P<0.05).Conclusion RECK,MMP-2,and bcl-2 proteins are expressed differently in the gingival tissue of children with gingival tumors.The positive expression rate of RECK is low,while the expression of MMP-2 and bcl-2 proteins is high.Their expression is closely related to recurrence,which can provide some guidance for improving early treatment work.