乙醇对干酪乳酪杆菌Zhang甲基化转移酶突变体的影响

Effect of Ethanol on Methylated Transferase Mutant of Lacticaseibacillus casei Zhang

乳酸菌应用于酒精饮料的生产时会面临乙醇胁迫,增强其乙醇耐受性在工业育种中显得尤为重要。分离自内蒙古传统酸马奶的干酪乳酪杆菌Zhang,是一株发酵性能优良的益生菌。本研究以干酪乳酪杆菌Zhang及其甲基化转移酶突变体为研究对象,采用液相色谱-质谱联用技术对其在10%乙醇胁迫下生长12 h的差异代谢物进行系统分析,目的是探明乙醇胁迫下DNA甲基化对干酪乳酪杆菌代谢物的影响。在甲基化转移酶突变体和野生型之间共鉴定得到199个差异代谢物,进一步富集分析显示差异代谢物主要在氨基酸代谢、ABC转运体、双组分系统以及丙酮酸代谢途径显著富集。这些研究结果对乳酸菌工业育种具有重要意义。

Lactic acid bacteria will face ethanol stress when used to produce alcoholic beverages,so it is particularly important to enhance the ethanol stress tolerance of lactic acid bacteria in industrial breeding.Lacticaseibacillus casei Zhang(L.casei Zhang),isolated from traditional koumiss in Inner Mongolia,is a probiotic strain with excellent fermentation performance.In this study,the differential metabolites between L.casei Zhang and its methylated transferase mutant were systematically analyzed by Liquid Chromatograph Mass Spectrometer(LC-MS)under 10% ethanol stress for 12 h.The aim of this study was to investigate the effects of DNA methylation on metabolites of L.casei Zhang under ethanol stress.t A total of 199 differential metabolites were identified between the methylated transferase mutant and the wild-type.Further enrichment analysis showed that the differential metabolites were mainly enriched in amino acid metabolism,ABC transporters,two-component system and pyruvate metabolism pathways.These findings have important implications for industrial breeding of lactic acid bacteria.