茶多酚/玉米醇溶蛋白/明胶同轴静电纺丝膜的制备及其保鲜性能

Preparation and Preservation Properties of Tea Polyphenol/Zein/Gelatin Coaxial Electrospinning Film

目的:制备负载茶多酚的蛋白纳米纤维膜作为金枪鱼活性包装膜,探究该包装膜的特征及保鲜性能。方法:采用同轴静电纺丝技术制备载有不同质量浓度(10,20,30 mg/mL)芯材茶多酚(TP)的玉米醇溶蛋白/明胶(ZG)纤维膜,通过扫描电镜、红外光谱、X射线衍射、差示扫描量热法、亲疏水性、抗菌及抗氧化性测试分析纤维膜的形态、特性及其对金枪鱼的保鲜性能。结果:ZG,TP1/ZG,TP2/ZG和TP3/ZG膜的平均纤维直径分别为(810±240),(930±270),(880±150),(850±610)nm。TP在TP/ZG同轴纳米纤维中未显著改变ZG膜的化学结构和晶体结构。各TP/ZG膜在小于130℃附近有一个类似ZG的热吸收峰。TP1/ZG和TP2/ZG、TP3/ZG的60 s接触角分别为(105.89±5.14)°,(91.32±5.11)°,(73.36±4.20)°。TP/ZG膜的热性能和水接触角随TP浓度的增加而降低。TP赋予了TP/ZG膜良好的抗菌和抗氧化性能,对水产品腐败希瓦氏菌具有显著的抑制作用。TP/ZG能有效抑制金枪鱼鱼片在4℃贮藏条件下的菌落总数、pH值、总挥发性碱氮、脂肪氧化和质构劣变,可延长货架期3 d左右。结论:负载适量TP的ZG同轴纤维膜,具有良好的形貌、耐水性、抗菌性、抗氧化性及保鲜性能,可作为一种新型的抗菌抗氧化食品包装材料。

Objective:Protein fiber films loaded with tea polyphenols were obtained as the active packaging films for tuna,and the characteristics and preservation properties of the packaging film were explored.Method:Zein/gelatin(ZG)fiber films loaded with different concentration core materials of tea polyphenols(10,20,30 mg/mL)was prepared by coaxial electrospun technology.The morphology and characteristics of the fiber film were analyzed by scanning electron microscopy,infrared spectroscopy,X-ray diffraction,differential scanning calorimetry,hydrophilicity and hydrophobicity,antibacterial and antioxidant tests,and preservation properties for tuna was analyzed.Results:The average fiber diameters of ZG,TP1/ZG,TP2/ZG and TP3/ZG films were(810±240),(930±270),(880±150)and(850±610)nm,respectively.TP in TP/ZG coaxial nanofibers did not significantly change the chemical structure and crystal structure of the ZG film.Each TP/ZG film has a ZG-like heat absorption peak near less than 130℃.The 60 s contact angles of TP1/ZG,TP2/ZG and TP3/ZG are(105.89±5.14)°,(91.32±5.11)°and(73.36±4.20)°,respectively.The thermal properties and water contact angles of TP/ZG films decreased with increasing TP concentration.TP endowed TP/ZG membrane with good antibacterial and antioxidant properties,and has a significant inhibitory effect on Shewanella putrefaciens in aquatic products.TP/ZG can effectively inhibit the total viable counts,pH value,volatile base nitrogen,fat oxidation and texture deterioration of tuna fillets stored at 4℃,and can prolong the shelf life of tuna for 3 days.Conclusion:ZG coaxial fiber film loaded with appropriate TP possessed good morphology,water resistance,antibacterial,antioxidant and preservation properties,which can be used as a new type of antibacterial and antioxidant food packaging material.