核桃叶及其乙醇提取物化学成分分析

Chemical composition analysis of walnut leaves and their ethanol extracts

[目的]探明核桃叶的化学成分。[方法]利用固相微萃取(SPME)技术结合气相色谱—质谱法(GC-MS),以及超高效液相色谱—四极杆—静电场轨道阱高分辨质谱联用技术(UPLC-Q-exactive),分别对核桃叶中的挥发性成分和乙醇提取物的化学成分进行鉴定分析。[结果]核桃叶中含88个挥发性成分,主要为萜类化合物,其中倍半萜占挥发性成分含量的78.35%;乙醇提取物中功能成分达30种,其中多酚类物质20种(黄酮类12种),苯丙素类4种,五环三萜类3种,必需氨基酸2种,三羧酸类化合物1种。[结论]SPME-GC-MS和UPLC-Q Exactive技术的联合使用可以系统、准确、快速地测定和分析核桃叶的化学成分。

[Objective]This study aimed to investigate the chemical constituents of walnut leaves.[Methods]The volatile components of walnut leaves were analyzed by head-space solid micro-extraction and coupled with GC-MS.The chemical composition of ethanol extract was identified using ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high resolution mass spectrometry(UPLC-Q-Exactive).[Results]88 kinds of volatile compounds were identified in walnut leaves,mainly terpenoids,of which sesquiterpenoids account for 78.35%.30 kinds of active components were identified from ethanol extracts,including twenty kinds of polyphenols(twelve kinds of flavonoids),four kinds of phenylpropanoids,three kinds of pentacyclic triterpenoids,two kinds of essential amino acids and 1 tricarboxylic acid compounds in the ethanol extract in walnut leaves.[Conclusion]The combined use of SPME-GC-MS and UPLC-Q Exactive technology can determine and analyze the chemical composition of walnut leaves systematically,accurately and rapidly.