指纹图谱和化学模式识别研究黄芪的不同基原和生长方式

Fingerprint and Chemical Pattern Recognition Study on Astragali Radix of Different Botanical Origins and Growth Patterns

目的:基于化学组分信息探究不同基原、生长方式的黄芪药材的质量差异。方法:采用高效液相色谱-蒸发光散射检测技术(HPLC-ELSD)建立指纹图谱分析方法,对收集的28批不同基原、不同生长方式的黄芪样品进行相似度评价和化学模式识别分析。结果:建立的黄芪HPLC指纹图谱共鉴定出11个共有峰,聚类分析将28批样品分为蒙古黄芪和膜荚黄芪两类,其中蒙古黄芪明显分为野生/仿野生黄芪和移栽黄芪两类。正交偏最小二乘法判别分析(OPLS-DA)筛选出5个野生/仿野生黄芪与移栽黄芪的差异性成分。结论:本研究建立的黄芪HPLC-ELSD指纹图谱结合化学模式识别方法,较为全面地反映出黄芪药材化学成分的总体质量特征,可区分不同基原和不同生长方式的黄芪药材,可为黄芪药材质量控制提供参考。

Objective:Based on the information of chemical components,the differences of Astragali Radix samples form different botanical origins and growth patterns were studied.Methods:A fingerprint analysis method was established by high performance liquid chromatography with evaporative light scattering detection(HPLC-ELSD),and similarity evaluation and chemical pattern recognition analysis were carried out on 28 batches of Astragali Radix samples from different botanical origins and growth patterns.Results:The HPLC fingerprints of Astragali Radix were established,and 11 common peaks were identified.Cluster analysis divided the 28 batches of samples into two categories:Astragalus mongholicus and Astragalus membranaceus,among which the Astragalus mongholicus samples were obviously divided into wild/wild-simulated and cultivated.Five components differensiating wild/wild-simulated Astragali Radix and cultivated Astragali Radix were selected by orthogonal partial least squares discriminant analysis(OPLS-DA).Conclusion:The HPLC-ELSD fingerprints of Astragali Radix combined with the chemical pattern recognition method established in this study can comprehensively reflect the overall quality characteristics of the chemical components of Astragali Radix,and can distinguish Astragali Radix from different botanical origins and growth patterns,which can provide reference for the quality control of Astragali Radix.