慢性肝病患者血清白介素-7的检测及其临床意义

Serum Interleukin-7 in Patients with Chronic Liver Disease

目的 检测白介素-7(IL-7)在慢性肝病患者不同阶段血清及尿液中的水平及,探讨其临床意义,为肝病患者的治疗提供参考。方法 收集30例健康受试者(A1组)、56例慢性肝炎患者(A2组)、37例肝硬化患者(A3组)血清和尿液样本,共计147份,其中A1组血清样本13份、尿液样本17份,A2组血清样本24份、尿液样本40份,A3组血清样本24份、尿液样本29份。采用ELISA法检测各组样本IL-7水平,采用全自动生化仪检测各组血清样本白蛋白(ALB)水平,采用肝脏弹性测量和肝脏脂肪变性定量检测设备检测各组受试者肝脏弹性硬度值(kPa值),并分析IL-7水平与ALB、kPa值的相关性。结果 血清IL-7水平A1组>A2组>A3组,A1组与A3组比较差异有统计学意义(P<0.05),A2组与A1组、A3组比较差异无统计学意义(P>0.05);尿液IL-7水平A1组>A2组>A3组,但差异均无统计学意义(P>0.05)。血清IL-7水平与ALB呈显著正相关(r=0.226,P=0.041),与肝弹性硬度kPa值呈显著负相关(r=-0.258,P=0.045);尿液IL-7水平与血清ALB呈显著正相关(r=0.221,P=0.046)。结论 随着慢性肝病的进展,血清IL-7水平呈下降趋势;IL-7水平与肝纤维化密切相关,可能具有延缓或抑制肝纤维化的潜力。

Objective To investigate the levels and clinical significance of interleukin-7(IL-7)in serum and urine of patients with chronic liver disease at different stages,and to provide some references for the treatment of patients with liver disease.Methods A total of 147 serum and urine samples were collected from 123 participants:30 healthy individuals(Group A1),56 patients with chronic hepatitis(Group A2),and 37 patients with cirrhosis(Group A3).The samples were divided into 30 copies of Group A1(13 serum samples and 17 urine samples),64 copies of Group A2(24 serum samples and 40 urine samples),and 53 copies of Group A3(24 serum samples and 29 urine samples).The ELISA method was used to detect the IL-7 levels of the samples in each group;the fully automated biochemistry instrument was used to detect the albumin(ALB)levels of the serum samples in each group;the liver elasticity measurement and liver steatosis quantitative detection equipment was used to detect the liver elasticity hardness values(kPa value)of the subjects in each group and to analyze the correlation between the IL-7 levels and the ALB and kPa values.Results The serum IL-7 levels varied in the three groups(group A1>group A2>group A3);the difference between Group A1 and Group A3 was statistically significant(P<0.05)and the difference between group A2 and group A1 and group A3 was not statistically significant(P>0.05).The urinary IL-7 levels varied in the three groups(group A1>group A2>group A3),but none of the differences was statistically significant(P>0.05).Further studies found that the serum IL-7 levels were significantly and positively correlated with the ALB levels(r=0.226,P=0.041);the serum IL-7 levels were significantly and negatively correlated with the liver elasticity hardness kPa values(r=-0.258,P=0.045);the urinary IL-7 levels showed a significant positive correlation with the serum ALB levels(r=0.221,P=0.046).Conclusion The serum IL-7 levels tended to decrease with the progression of chronic liver disease.The IL-7 levels could be closely associated with liver fibrosis and might have the potential to delay or inhibit liver fibrosis.