MXD3表达水平与前列腺癌患者预后和免疫浸润的相关性研究

The Correlation of MXD3 Expression Level and Prognosis and Immune Infiltration in Patients with Prostate Cancer

目的 探究MAX二聚化蛋白3(MXD3)在前列腺癌中的表达及与患者预后和免疫浸润的相关性。方法从TCGA数据库获取前列腺癌样本和正常样本的RNA测序数据和临床信息,比较肿瘤和相邻正常组织之间的MXD3表达水平。进行Kaplan-Meier生存分析,评估MXD3表达水平的预后价值和MXD3和临床特征之间的关联。采用GO和KEGG进行基因富集分析,使用ESTIMATE算法分析前列腺癌中MXD3表达与免疫细胞浸润之间的相关性。通过qRT-PCR和蛋白质印迹验证前列腺癌组织中的MXD3 mRNA和蛋白质表达水平。结果MXD3的表达水平在前列腺癌中显著高于正常前列腺组织,其高表达与不良预后显著相关,而且MXD3表达水平与肿瘤的临床分期呈正相关(P<0.05或P<0.001)。GO功能和KEGG通路富集分析结果显示MXD3与前列腺癌细胞的分裂增殖密切相关。MXD3的表达与调节性T细胞和滤泡辅助T细胞的免疫细胞浸润水平呈正相关(P<0.001和P<0.05),而与肥大细胞和CD4^(+)T细胞呈负相关(P<0.01和P<0.001)。与癌旁正常组织相比,MXD3在前列腺癌中mRNA和蛋白质表达显著增加(P<0.01)。结论 MXD3在前列腺癌中高表达,与前列腺癌患者不良预后有关,参与前列腺癌的分裂增殖并与免疫浸润相关,是潜在的预后生物标志物和免疫靶点。

Objective To investigate the expression of MAX dimerization protein 3(MXD3)in prostate cancer(PCa)and its correlation with patient prognosis and immune infiltration.MethodsRNA sequencing data and clinical information of PCa samples and normal samples were obtained from TCGA database to compare the MXD3 expression levels between tumors and adjacent normal tissues.Kaplan-Meier survival analysis was performed to assess the prognostic value of MXD3 expression levels and the association between MXD3 and clinical features.Gene enrichment analysis was performed using GO and KEGG,and the correlation between MXD3 expression and immune cell infiltration in prostate cancer was analyzed using the ESTIMATE algorithm.The expression levels of MXD3 mRNA and protein in PCa tissues were verified by qRT-PCR and protein blotting.Results The expression level of MXD3 was significantly higher in prostate cancer than in normal prostate tissues,and its high expression was significantly correlated with poor prognosis,and the expression level of MXD3 was positively correlated with the clinical stage of the tumor(P<0.05 or P<0.001).GO function and KEGG pathway enrichment analysis showed that MXD3 was closely related to the division and proliferation of prostate cancer cells.The expression of MXD3 was positively correlated with immune cell infiltration levels of regulatory T cells and follicular helper T cells(P<0.001 and P<0.05),whereas it was negatively correlated with mast cells and CD4^(+)T cells(P<0.01 and P<0.001).The mRNA and protein expression of MXD3 was significantly increased in prostate cancer compared with adjacent tissues(P<0.01).ConclusionMXD3,highly expressed in prostate cancer,can be associated with poor prognosis in patients with prostate cancer.MXD3 participates in the division and proliferation of prostate cancer,and is associated with immune infiltration.MXD3 could serve as a potential prognostic biomarker and immune target.