COL7A1和LAMC2在口腔鳞状细胞癌中的表达及预后意义

Expression and Prognostic Significance of COL7A1 and LAMC2 in Oral Squamous Cell Carcinoma

目的:探讨口腔鳞状细胞癌(oral squamous cell carcinoma, OSCC)中Ⅶ胶原蛋白α-1链(typeⅦCollagen α-1 chain, COL7A1)和层粘连蛋白γ-2(laminin γ-2,LAMC2)的表达水平,明确COL7A1和LAMC2的表达水平与OSCC患者临床病理特征及预后的相关性。方法:应用免疫组织化学法检测108例OSCC组织和55例癌旁组织中COL7A1和LAMC2的表达,分析COL7A1和LAMC2表达水平与OSCC患者临床病理特征及预后的关系;采用实时荧光定量聚合酶链反应和Western blot检测OSCC细胞系中COL7A1和LAMC2的表达量;应用生物信息学数据库进行验证。结果:OSCC组织中COL7A1和LAMC2的表达均显著高于癌旁组织(P<0.001),且两者的表达呈正相关(r=0.655,P<0.001);COL7A1的表达与T分期、TNM分期及淋巴结转移有关(P<0.05),LAMC2的表达与TNM分期和淋巴结转移有关(P<0.05);与正常口腔角质细胞相比,COL7A1和LAMC2在OSCC细胞系中均呈过表达(P<0.05);生存分析显示,COL7A1和LAMC2高表达组的总生存期均短于低表达组(P<0.05);多因素Cox回归分析表明COL7A1和LAMC2是影响OSCC患者预后的独立危险因素(P<0.05)。结论:COL7A1和LAMC2在OSCC中表达增高,与肿瘤的TNM分期以及转移有关,对OSCC患者的预后评估具有一定的参考价值。

Objective:To investigate the expression of typeⅦCollagenα-1 chain(COL7A1)and lamininγ-2(LAMC2)in oral squamous cell carcinoma,and to clarify the correlation between the expression of COL7A1 and LAMC2 and clinical parameters and prognosis in OSCC patients.Methods:Immunohistochemistry was used to detect the expression of COL7A1 and LAMC2 in 108 cases of OSCC tissues and 55 cases of adjacent tissues,and to analyze the correlation between COL7A1 and LAMC2 and the clinicopathological features and prognosis of OSCC patients.The expression levels of COL7A1 and LAMC2 in OSCC cell lines were detected by real-time fluorescence quantitative PCR and western blotting.Finally,the bioinformatics database was used for verification.Results:The expressions of COL7A1 and LAMC2 in OSCC tissues were significantly higher than those in adjacent tissues(P<0.001),and the expressions of COL7A1 and LAMC2 were positively correlated(r=0.655,P<0.001).The expression of COL7A1 was related to T stage,TNM stage,and lymph node metastasis(P<0.05),and that of LAMC2 was related to TNM stage and lymph node metastasis(P<0.05).Compared with normal oral keratinocytes,COL7A1 and LAMC2 were overexpressed in OSCC cell lines(P<0.05).Kaplan-Meier analysis showed that the overall survival time of the high expression group of COL7A1 and LAMC2 was shorter than that of the low expression group(P<0.05).Multiple Cox regression analysis showed that COL7A1 and LAMC2 was an independent risk factor for OSCC patients(P<0.05).Conclusion:The increased expression of COL7A1 and LAMC2 in OSCC has certain reference value for the prognosis evaluation of patients with oral squamous cell carcinoma,and is related to the TNM stage and metastasis of the tumor.