封育对荒漠草原真菌群落的影响

Effects of sealing on fungal communities in desert grasslands

【目的】评估封育对荒漠草原真菌群落的影响,并阐述真菌群落在土壤中的作用。【方法】采用野外调查取样的方法,以封育14年的荒漠草原和相邻自由放牧荒漠草原为研究对象,基于内转录间隔区(ITS)技术与液质联用(LC-MS)技术研究封育对荒漠草原真菌群落的影响。【结果】封育增加了荒漠草原子囊菌门(Ascomycota)的相对丰度,降低了担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)和unclassified_k__Fungi的相对丰度,显著改变了真菌群落的β多样性。此外,封育处理下土壤养分含量和植物生物量高于放牧处理。封育处理下真菌群落的内部联系更加紧密,主要关系为相互竞争,而放牧处理下真菌群落内部关系主要是互利共生。真菌群落与土壤总钾及植物生物量之间相关性较强。土壤差异真菌群落多与代谢物显著正相关。【结论】封育改变了荒漠草原真菌群落丰富度,增加了植物地上与地下部分生物量。

【Objective】Assess the response of closure to fungal communities and to describe the role of fungal communities in the soil.【Methods】A field survey was conducted to investigate the effect of sealing on the fungal communities of desert grasslands based on the internal transcribed spacer(ITS)technique and liquid chromatography-mass spectrometry(LC-MS),using 14 years of sealing and adjacent free-grazing desert grasslands as the study objects.【Results】The results showed that sealing increased the community abundance of Ascomycota and decreased the community abundance of Basidiomycota,Mortierellomycota,and unclassified_k_Fungi,significantly altered the beta diversity of the fungal community.In addition,soil nutrient content and plant biomass were higher under the sealing treatment than under the grazing treatment.The fungal communities were more closely linked internally under closed conditions,with the main relationships being competitive with each other,whereas under grazing conditions the fungal communities were mainly in a mutually beneficial symbiotic relationship.A strong correlation between fungal communities and total soil potassium and plant biomass.Soil differential fungal communities were mostly significantly and positively correlated with metabolites.【Conclusion】Sealing altered fungal community richness and increased plant biomass above and below ground.