假小链双歧杆菌比较基因组学分析

Comparative genomics analysis of Bifidobacterium pseudocatenulatum

该研究对分离自中国、日本和美国的104株假小链双歧杆菌进行比较基因组学分析。结果表明,在基因组特征上,日本分离株的编码序列数量显著高于中国和美国分离株(P<0.01)。基于核心基因构建的系统发育树和平均核苷酸一致性聚类热图发现分离自相同国家的菌株更相似。功能基因注释结果进一步表明分离地会影响菌株的功能基因分布,碳水化合物活性酶注释结果显示15个碳水化合物活性酶存在地域性差异;经耐药基因和毒力因子数据库注释发现中国分离株携带的耐药基因和黏附型毒力因子的数量显著高于日本分离株;中国和日本分离株携带复制、重组和修复及噬菌体相关可移动遗传元件数量均与美国分离株存在差异。该研究发现分离地会影响假小链双歧杆菌的功能基因,不同分离地假小链双歧杆菌存在差异功能基因。研究结果可为解析假小链双歧杆菌的功能差异提供理论依据。

In order to analyze the differences in functional genes of Bifidobacterium pseudocatenulatum.Comparative genomics analysis was performed on 104 strains of Bifidobacterium pseudocatenulatum isolated from China,Japan and America.The results showed that the number of coding sequences of Japanese isolates were significantly higher than Chinese and American isolates in genome level(P<0.01).Phylogenetic tree based on core gene and average nucleotide identity clustering heat map showed that strains isolated from the same country were more similar.The results of functional gene annotation further showed that the isolation could affect the functional genes distribution of strains.The annotation of carbohydrate active enzymes revealed that 15 carbohydrate active enzymes had regional differences.The number of antibiotic resistance genes and adhesion virulence factors carried by Chinese isolates were more abundant than Japanese isolates by the annotation of antibiotic resistance genes database and virulence factors database.The number of Replication\recombination\repair and phage associated mobile genetic elements carried by the Chinese and Japanese isolates were different from American isolates.In this study,it was found that the location of strains will affect the functional genes of Bifidobacterium pseudocatenulatum,and Bifidobacterium pseudocatenulatum in different locations have different functional genes.This study provides theoretical basis for the analysis of the functional differences of Bifidobacterium pseudocatenulatum.