基于金纳米粒子荧光探针可视化快速检测复杂样品中氰化物

Visualized and rapid detection of cyanide in complex samples using fluorescence probes based on gold nanoparticles

该研究利用水溶性的荧光素异硫氰酸酯(fluorescein isothiocyanate,FITC)覆盖石墨烯负载金纳米粒子复合材料,使探针分子FITC的荧光猝灭。然后,通过向体系中加入氰化物,氰根离子(CN-)对金纳米粒子的强络合作用形成稳定的络合物Au(CN)3-x而使探针分子荧光逐渐恢复,以此实现氰化物的快速检测。对试验条件溶液pH值、反应时间、干扰离子进行了优化。试验结果表明,在H_(2)O_(2)存在下,基于FITC-Au NPs荧光探针对CN-最低检测限达到了0.032μmol/L,在0.05~80μmol/L内具有良好的线性关系。利用FITC-Au NPs传感探针的高稳定性和选择性实现了复杂样品中CN-的快速检测。这种简单、快速、经济的荧光传感系统在实际样品中阴离子的检测方面显示出巨大的实用潜力。

This study utilized water-soluble fluorescein isothiocyanate(FITC)to cover graphene loaded gold nanoparticle composites,and the fluorescence of the probe molecule FITC was quenched.Then,by adding cyanide to the system,the strong complexation of cyanide ions(CN-)with gold nanoparticles formed a stable complex Au(CN)3-x,gradually restoring the fluorescence of the probe molecules,thereby achieving rapid detection of cyanide.The pH value of the experimental solution,reaction time,and interfering ions were optimized.The experimental results indicated that the FITC-Au NPs fluorescence probe had a minimum detection limit of 0.036μmol/L for CN-in the presence of an H_(2)O_(2)environment.There was a good linear relationship within the 0.05-80μmol/L concentration range.The high stability and selectivity of FITC-Au NPs probes were utilized to achieve rapid detection of CN-in complex samples.This simple,fast,and economical fluorescence sensing system shows great practical potential in the detection of anions in actual samples.