维药祛湿健骨方防治绝经后骨质疏松症的作用机制

Mechanism of Uyghur medicine Qushi Jiangu recipe in the prevention and treatment of postmenopausal osteoporosis

目的 基于钙磷调节研究维药祛湿健骨方防治绝经后骨质疏松症的作用及其机制。方法 70只SD大鼠随机分为7组,正常对照组(Control组)、假手术组(Sham组)、手术造模组[OVX组、阳性对照组(EVT组)、低剂量组(QSJG-L组)、中剂量组(QSJG-M组)、高剂量组(QSJG-H组)],每组10只,手术造模组行OVX手术;EVT组给予补佳乐(Estradiol Vital GmbH)0.09 mg/(kg·d),按照QSJG-L组0.175 g/(kg·d)、QSJG-M组0.35 g/(kg·d)、QSJG-H组0.7 g/(kg·d)进行灌胃,其余组均给予等量蒸馏水,各组同时灌胃9周。Micro-CT检测骨密度,HE观察病理变化,ELISA检测血清雌激素(E2)、血钙(Ca)、血磷(P)等含量;免疫组化法检测肾脏1α羟化酶含量。结果 与OVX组相比,QSJG-H组骨密度提高(P<0.01);骨体积百分比、骨表面积、骨表面密度、骨小梁数量显著提高,骨表面积体积比、骨小梁分离度显著下降(P<0.01);HE显示QSJG-H组骨小梁间连接更佳,更为完整,粗细程度均匀,少见骨小梁断裂和骨折痕迹;E2、Ca、1,25(OH)2D3(P<0.01)含量升高,P、PTH的含量显著降低(P<0.01);肾脏1α-羟化酶表达明显升高(P<0.01)。结论 维药祛湿健骨方能够促进肾脏1α-羟化酶的活化升高1,25(OH)2D3水平,抑制PTH,从而调节钙磷代谢发挥防止骨质疏松作用。

Objective To investigate the effect and mechanism of Uyghur medicine Qushi Jiangu Recipe on the prevention and treatment of postmenopausal osteoporosis based on calcium and phosphorus regulation.Methods Seventy SD rats were randomly divided into 7 groups:normal control group(Control group),sham operation group(Sham group),and surgical modeling groups including OVX group,positive control group(EVT group),low-dose group(QSJG-L group),medium-dose group(QSJG-M group),and high-dose group(QSJG-H group),with 10 rats in each group.OVX was performed in the surgical modeling groups.Rats received Bujiale(Estradiol Vital GmbH)0.09 mg/(kg·d)in EVT group,0.175 g/(kg·d)in QSJG-L group,0.35 g/(kg·d)in QSJG-M group,and 0.7 g/(kg·d)in QSJG-H group,respectively,by gavage.Rats in other groups received the same amount of distilled water.The treatment lasted for 9 weeks.Micro-CT was used to detect bone mineral density.HE was used to observe pathological changes.ELISA was used to detect serum estrogen(E 2),blood calcium(Ca),blood phosphorus(P),and other contents.Immunohistochemistry was used to detect renal 1αhydroxylase content.Results Compared to those in OVX group,bone mineral density(BMD)increased in QSJG-H group(P<0.01).Bone volume percentage,bone surface area,bone surface density,and trabecular number significantly increased.Bone surface area volume ratio and trabecular separation significantly decreased(P<0.01).HE result showed that trabecular interconnection was better,more complete,uniform thickness,and rare trabecular fracture traces in QSJG-H group.Serum E 2,Ca,1,25(OH)2D 3 content increased(P<0.01).P and PTH contents significantly decreased(P<0.01).Renal 1α-hydroxylase expression significantly increased(P<0.01).Conclusion Uyghur medicine Qushi Jiangu recipe promotes the activation of renal 1α-hydroxylase to increase the level of 1,25(OH)2D 3,inhibits PTH,and thus regulates calcium and phosphorus metabolism to play a role in preventing osteoporosis.