全反式维甲酸对肝纤维化氧化应激损伤自然杀伤细胞的保护作用

Protective effect of all-trans retinoic acid on natural killer cells damaged by oxidative stress in liver fibrosis

目的:探讨全反式维甲酸(ATRA)对肝纤维化氧化应激损伤NK细胞功能的保护作用。方法:采用CCl_(4)制备肝纤维化小鼠模型,通过生化及病理检测评价小鼠肝脏受损及纤维化程度,观察小鼠肝组织NK细胞变化及氧化应激损伤;体外采用H_(2)O_(2)诱导的NK92细胞株氧化应激损伤模型评价ATRA对NK92细胞的保护作用。结果:ATRA可以改善CCl_(4)模型小鼠肝功能,抑制肝脏炎症及纤维化,增加肝脏SOD活性和GSH含量,促进肝内NK细胞比例和数量;体外实验证实ATRA可以保护H_(2)O_(2)诱导的NK细胞氧化损伤及凋亡。结论:ATRA可以改善CCl_(4)引起的肝损伤及纤维化,可能与其保护NK细胞氧化应激损伤有关。

Objective:To investigate the protective effect of all-trans retinoic acid(ATRA)on the function of NK cells damaged by oxidative stress in liver fibrosis.Methods:Mouse model with liver injury was established by carbon tetrachloride(CCl_(4)),biochemical and pathological assays were used to evaluate the degree of liver injury and fibrosis in mice,and changes of NK cells and oxidative stress injury in liver tissue of mice were observed.In vitro,NK92 cell lines damaged by H_(2)O_(2) oxidative stress were evaluated the protective effect of ATRA.Results:Liver inflammation and fibrosis were suppressed and liver function was improved in CCl_(4) model mice by ATRA treatment.ATRA could increase SOD activity and GSH content in liver tissue,which promoted the proportion and number of hepatic NK cells.ATRA could protect NK cells from oxidative damage and apoptosis induced by H_(2)O_(2) in vitro.Conclusion:ATRA can ameliorate liver injury and fibrosis induced by CCl_(4),which may be related to the inhibition of oxidative stress on NK cells.