超声波辅助低共熔溶剂提取荞麦壳中总黄酮的工艺研究

Study on the technology of extracting total flavonoids from buckwheat shell with ultrasonic-assisted deep eutectic solvent

以荞麦壳为原料,采用超声波辅助低共熔溶剂提取荞麦壳中总黄酮。实验对低共熔溶剂(DES)的种类、摩尔比、含水量进行了筛选,在此基础上优化了超声提取工艺条件。结果表明,用摩尔比为1∶2的氯化胆碱/乙二醇合成的低共熔溶剂,当DES含水量为30%、液料比为30∶1(mL∶g)、超声温度为70℃、超声时间为40min时,总黄酮提取得率为8.26mg·g^(-1),高于传统醇提得率。此工艺绿色温和,适用于荞麦壳中总黄酮的高效提取,研究为植物源废弃物中天然产物的提取提供了新技术。

Using buckwheat husk as raw material,total flavonoids in buckwheat hull were extracted by ultrasonic-assisted deep eutectic solvent.The experiment screened the types,molar ratios,and water content of the deep eutectic solvents(DES),and optimized the ultrasonic extraction process conditions on this basis.The results showed that the total flavonoid extraction rate was 8.26mg·g^(-1)using the deep eutectic solvent synthesized from choline chloride/ethylene glycol with a molar ratio of 1∶2,when the water content of DES was 30%,the liquid-solid ratio was 30∶1(mL∶g),the ultrasound temperature was 70℃,and the ultrasound time was 40 minutes,which was higher than the traditional alcohol extraction rate.This process is green and gentle,suitable for efficient extraction of total flavonoids from buckwheat husks,and provides a new technology for the extraction of natural products from plant-based waste.