高山火绒草类囊泡的提取、表征及其生物活性的检测

Extraction and characterization of the extracellular vesicles derived from Leontopodium Alpinum(Edelweiss)callus culture supernatant and evaluation of their biological efficacy in Vitro

研究高山火绒草类囊泡提取、表征及其生物活性。采用切向流澄清、浓缩工艺和柱层析方法从高山火绒草愈伤组织培养上清液中提取了高山火绒草类囊泡,分别测试高山火绒草类囊泡的细胞毒性、抗氧化能力、是否促进Col1a1蛋白的生成、是否抑制基质金属蛋白酶-1(MMP-1)的表达和促细胞迁移能力。结果表明,从高山火绒草愈伤组织培养上清液中能够获得高纯度的高山火绒草类囊泡,500 μg/mL高山火绒草类囊泡具有显著的抗氧化、促Col1a1蛋白生成和抑制基质金属蛋白酶-1(MMP-1)表达,促细胞迁移等方面的生物活性。

The study aims to study the extraction,characterization and biological activity of vesicles from Leontopodium alpinum.The sacs of Leontopodium Alpinum were extracted from the supernatant of Leontopodium Alpinum callus culture using tangential flow clarification,concentration process,and column chromatography.The cytotoxicity,antioxidant capacity,promotion of Col1a1 protein production,inhibition of matrix metalloproteinase-1(MMP-1)expression,and promotion of cell migration ability of Leontopodium Alpinum vesicles were tested.The results showed that high-purity thylakoids could be obtained from the supernatant of the callus culture of Leontopodium Alpinum.The thylakoids of Leontopodium Alpinum had significant antioxidant,Col1a1 protein production,and matrix metalloproteinase-1(MMP-1)expression inhibition,cell migration promotion,and other biological activities at the concentration of 500μg/mL.