液相色谱质谱法分析多菌灵含量的溶剂效应

Analysis of Solvent Effect of Carbendazim by Liquid Chromatography-mass Spectrometry

本研究通过采用液相色谱质谱法(Liquid chromatography-mass spectrometry,LC-MS)分析溶剂效应对多菌灵含量测定的影响。样品经0.1%乙酸乙腈提取,选取硫酸镁、乙二胺-N-丙基硅烷、十八烷基硅烷作为净化剂,测定不同溶剂下多菌灵的残留量。试验结果表明,溶剂组分对多菌灵的峰高、峰面积以及半峰宽均有不同影响,当溶剂为流动相A∶B比例9∶1和8∶2时,多菌灵的回收率和峰型较好,之后随着有机相比例增加,回收率相对减少,峰型逐渐变宽;当用纯乙腈进样时,发现在目标峰前面有一个干扰峰,目标峰峰型较差。该结果可以用于准确测定农产品中多菌灵含量,为从事农药残留检测的专业技术人员提供参考。

In this study,Liquid chromatography-mass spectrometry(LC-MS)was used to analyze the influence of solvent effect on the determination of carbendazim content.The samples were extracted with 0.1%acetonitrile acetate,and magnesium sulfate,ethylenediamine-n-propyl silane and octadecyl silanewere selected as purifying agents to determine the residual amount of carbenda-zim in different solvents.The results showed that the solvent components had different effects on the peak height,peak area and half-peak width of carbendazim.When the solvent was mobile phase A∶B ratio of 9∶1 and 8∶2,the recovery rate and peak shape of carbendazim were better,and then with the increase of organic phase ratio,the recovery rate decreased and the peak shape became wider.When pure acetonitrile was injected,an interference peak was found in front of the target peak,and the target peak shape was poor.The results can be used for the accurate determination of carbendazim content in agricultural products,and provide a reference for professional and technical personnel engaged in pesticide residue detection.