摘要
The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from mammals and used for genetic analysis and complex genetic manipulation(Elling et al.,2011;Yang et al.,2012).The diploidization of haploid cells is an interesting biological phenomenon and has been widely studied,but the mechanism of diploidization has not yet been fully resolved(Sun et al.,2020).The zebrafish is an excellent vertebrate animal model used in biological studies(Huang et al.,2022).Researchers have isolated many cell lines from zebrafish,such as the zebrafish fibroblast line ZF4(Driever and Rangini,1993).However,there have been no reports regarding stable haploid cell lines of zebrafish,and whether diploidization occurs in zebrafish is unclear.
基金
supported by the National Key Research and Development Program of China(2019YFA0109900 and 2018YFA0801003)
CAS Center for Excellence in Molecular Cell Science(2021DF06)
Shanghai Municipal Science and Technology Major Project.
