模拟干旱胁迫下5份野生披碱草属植物萌发期抗旱性评价

Evaluation of Drought Resistance of Five Wild Elymus Plants during Germinatiunder Simulated Drought Stress

【目的】探究5份野生披碱草属种质材料萌发期的抗旱性,以期筛选出抗旱性较好的种质用于进一步种质抗旱研究。【方法】用PEG-6000作为模拟干旱处理试剂,将其配置成0%(蒸馏水)、5%、10%、15%、20%和25%6个浓度梯度的溶液,将不同浓度的溶液加入培养皿中,测定5份材料的发芽势、发芽率、萌发指数、活力指数、根长和芽长。【结果】随着PEG浓度的上升,5份材料的各指标均呈下降趋势,当浓度达到25%时各指标均达到最低值,E02、E04、E05材料已经不能萌发,而E01、E03虽然仍能萌发但萌发能力显著下降。【结论】5%浓度的PEG对材料发芽势和发芽率有一定的促进作用。5份材料的萌发期抗旱性排名为:E01>E05>E02>E03>E04。

【Objective】To investigate the drought resistance of 5 wild Elymus germplasm materials during the germination period,in order to screen out better germplasm for further research on drought resistance.【Methods】With PEG-6000 as the simulated drough treatment reagent,it is configured into 6 concentratio gradient sdutions of 0%(distilled water)、5%、10%、15%、20%and 25%.Different concentrations of solutions were added to culture dishes to determine the germination potential,germination rate,germination index,vitality index,root length and shoot length of the five materials.【Results】As the PEG concentration increased,all the indicators of the five materials showed a downward trend.When the concentration reached 25%,all the indicators reached their lowest values.E02,E04,and E05 materials could no longer germinate,while E01 and E03 could still germinate,but their germination ability was significantly insufficient.【Conclusion】5%concentration of PEG had a certain promoting effect on the germination potential and germination rate of the materials,and the drought resistance of the five materials during the germination period was ranked as follows:E01>E05>E02>E03>E04.