茶树组织培养技术与应用研究进展

Advance in Tissue Culture Techniques and Applications of Tea Plant(Camellia sinensis)

茶树是多年生异花授粉植物,具有较高的经济价值和饮用价值。植物组织培养技术为茶树种质资源离体保存、快速繁殖、遗传转化、多倍体育种、基因功能研究和次生代谢产物生产等提供了有效途径。本研究综述了茶树组织培养技术的发展概况和增殖、生根的主要影响因素,重点分析了组培过程中易出现的褐化、污染等问题及对策,并展望了茶树组织培养的主要应用前景,以期为茶树种质资源综合利用提供一定的参考。

Camellia sinensis,a pere nnial plant with cross-pollination characteristics,possesses significant economic and beverage value.The application of plant tissue culture technology offers an effective approach for various facets of tea plant cultivation,including rapid propagation,germplasm preservation,genetic transformation,polyploid breeding,gene function investigation,and the production of secondary metabolites.This review provides an overview of tea plant tissue culture technology,with a specific emphasis on factors influencing proliferation and rooting.Furthermore,it addresses prevalent challenges encountered during tea plant tissue culture,such as issues related to browning and contamination.Additionally,the review offers a comprehensive examination of the primary applications of tissue culture in tea plant cultivation.This study provides a reference for the improved utilization of tea plant germplasm resources.