柑橘皮活性成分橙皮苷结合有氧间歇运动对PM2.5致大鼠肺部损伤影响的研究

Study on the Effect of Hesperidin from Citrus Peel Combined with Aerobic Interval Exercise on PM2.5-Induced Lung Injury in Rats

橙皮苷(hesperidin,HES)是一种广泛存在于柑橘皮中的类黄酮物质,具有明显的抗炎、抗氧化以及器官保护作用,并且长期服用橙皮苷对人体健康有促进作用。本研究旨在探讨橙皮苷结合有氧间歇运动对PM_(2.5)致大鼠肺部损伤的治疗效果,及其在调节TLR4/MyD88/NF-κB信号通路方面的潜在机制。研究采用PM_(2.5)颗粒暴露大鼠模型,通过对比不同实验组大鼠肺组织中TLR4、My D88、p-NF-κB p65蛋白的表达水平,评估橙皮苷和有氧间歇运动的治疗效果。研究结果显示,与单独PM_(2.5)暴露组相比,橙皮苷治疗组和有氧间歇运动组均能显著降低TLR4、My D88、p-NF-κB p65的表达,减轻肺部炎症。其中,橙皮苷结合有氧间歇运动的联合干预效果最为显著。这些发现提示,橙皮苷结合有氧间歇运动能有效减轻PM_(2.5)引起的肺部损伤,其作用机制可能与其对TLR4/MyD88/NF-κB信号通路的调节有关。本研究为PM_(2.5)相关肺部疾病的预防和治疗提供了新的策略和理论依据。

Hesperidin(HES)is a flavonoid substance widely found in citrus peels,known for its significant antiinflammatory,antioxidant,and organ-protective effects.Long-term consumption of hesperidin also promotes human health.This study aimed to investigate the therapeutic effects of hesperidin combined with aerobic interval exercise on lung injury induced by PM_(2.5) in rats,and its potential mechanism in regulating the TLR4/MyD88/NF-κB signaling pathway.A PM_(2.5) particle exposure rat model was used,and the expression levels of TLR4,MyD88,and p-NF-κB p65 proteins in lung tissues of rats from different experimental groups were compared to assess the therapeutic effects of hesperidin and aerobic interval exercise.The results showed that both hesperidin treatment and aerobic interval exercise could significantly reduce the expression of TLR4,MyD88,and p-NF-κB p65 compared to the group exposed only to PM_(2.5),thereby alleviating lung inflammation.Among these,the combined intervention of hesperidin and aerobic interval exercise had the most significant effect.These findings suggest that hesperidin combined with aerobic interval exercise can effectively reduce lung injury caused by PM_(2.5),possibly related to its regulation of the TLR4/MyD88/NF-κB signaling pathway.This study provides new strategies and theoretical basis for the prevention and treatment of PM_(2.5)-related lung diseases.