HPLC法同时测定植物饮料中7种绿原酸

Simultaneous Determination of Seven Chlorogenic Acids in Plant Vegetable Beverages by HPLC

建立一种同时测定植物饮料中7种绿原酸的HPLC法:采用C18色谱柱,以乙腈+0.1%甲酸+0.1%乙酸作为流动相进行梯度洗脱,检测波长为327 nm,参比波长400 nm。7种绿原酸检出限为0.25 mg/L,在0.5~20μg/mL浓度范围内成线性关系,新绿原酸、绿原酸、隐绿原酸、异绿原酸B、1,5-二咖啡酰奎尼酸、异绿原酸A、异绿原酸C回收率分别为99.0%~108%、97.9%~101%、96.9%~102%、96.2%~106%、95.1%~96.7%、94.9%~99.6%、96.3%~108%。该方法简单、快捷、定性定量准确,可同时测定植物饮料中7种绿原酸。

An HPLC method was established for the simultaneous determination of seven chlorogenic acids in plant beverages,by using C18 chromatographic column,elutioned with acetonitrile+0.1%formic acid+0.1%acetic acid.The detection wavelength was 327 nm and the reference wavelength was 400 nm.The detection limit of seven chlorogenic acids was 0.25 mg/L,and the relationship was linear in the concentration range of 0.5~20µg/mL.The recoveries of neochloro genic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,1,5-dicaveoyl quinic acid,isochlorogenic acid A and isochlorogenic acid C were 99%~108%,97.9%~101%,96.9%~102%,96.2%~106%,95.1%~96.7%,94.9%~99.6%,96.3%~108%,respectively.The method was simple,fast,accurate in qualitative and quantitative,and could simultaneously determine seven kinds of chlorogenic acids in plant beverages.