略阳乌鸡和镇坪乌鸡的群体结构及遗传多样性分析

Population Structure and Genetic Diversity Analysis of Lueyang and Zhenping Black-Bone Chicken

略阳乌鸡和镇坪乌鸡是陕西省特有乌鸡群体,乌鸡肉营养丰富,深受消费者喜爱,具有巨大的经济价值。然而,至今尚未有研究系统阐明这两群体基因组特征。该研究采集20只略阳乌鸡、20只镇坪乌鸡样本进行全基因组重测序,并结合110只鸡公开数据构建SNP集合。通过群体结构分析发现中国地方鸡的聚类模式有明显的地理趋势,略阳乌鸡和镇坪乌鸡之间的遗传距离最近,表现出南北方家鸡遗传成分的混合。遗传多样性分析显示两乌鸡群体具有较高的观测杂合度、较快的连锁不平衡衰减以及较低的近交系数,遗传多样性较高。该研究加深了对陕西地方乌鸡群体遗传结构和多样性的认识,对略阳乌鸡和镇坪乌鸡种质资源保护具有一定参考意义。

Lueyang black-bone chicken(LBC)and Zhenping black-bone chicken(ZBC)are unique black-bone chicken populations native to Shaanxi Province.Due to their rich nutrition and popularity among consumers,they have substantial commercial value.However,few studies to date have comprehensively elucidated the genomic characteristics of these two populations.In this study,samples were collected from 20 Lueyang Black-Bone Chicken(LBCs)and 20 Zhenping Black-Bone Chicken(ZBCs)for whole genome re-sequencing.Additionally,SNP datasets were compiled by incorporating publicly available data from 110 chickens.Population structure analysis revealed that the clustering patterns of local chicken populations are related to their geographical location.LBC and ZBC exhibited the closest genetic distance and indicated a mixture of genetic components of Chinese northern and southern domestic chickens.Genetic diversity analysis showed that LBC and ZBC had relatively high observed heterozygosity,faster linkage disequilibrium decay,and lower inbreeding coefficients,indicating that they have rich genomic genetic diversity.This study deepens the understanding of the population structure of Shaanxi local black chicken breeds and has significant implications for the conservation of germplasm resources in LBC and ZBC.