针刺对桥本氏甲状腺炎大鼠肝脏氧化应激反应的影响

Effect of Acupuncture on Oxidative Stress in the Liver of Rats with Hashimoto's Thyroiditis

目的观察针刺对桥本氏甲状腺炎模型大鼠肝脏氧化应激反应的影响。方法将40只健康雌性大鼠随机分为空白组、模型组、西药组、针刺组,每组10只。除空白组外,其余三组制备桥本氏甲状腺炎模型,造模成功后第9周起模型组及空白组正常喂养,西药组予以硒酵母灌胃,针刺组予以针刺治疗。第14周,ELISA法检测血清TSH、FT3、FT4、TG-Ab、TPO-Ab、ALT含量;生化法测定肝脏组织中MDA、SOD、CAT含量,HE染色观察肝脏组织。结果与空白组相比,模型组大鼠血清TSH降低(P<0.05),FT3、FT4、TG-Ab、TPO-Ab、ALT及MDA、SOD、CAT水平上升(P<0.05);与模型组相比,针刺组及西药组大鼠血清TSH显著上升(P<0.05),FT3、FT4、TG-Ab、TPO-Ab、ALT及MDA、SOD、CAT含量明显降低(P<0.05);与西药组相比,针刺组大鼠血清TSH更高(P<0.05),FT3、FT4、TG-Ab、TPO-Ab、ALT及MDA、SOD、CAT水平更低(P<0.05)。与空白组相比,模型组肝脏组织有明显瘀堵;与模型组相比,针刺组及西药组肝瘀堵均有所改善,针刺组较西药组改善情况更佳。结论针刺能显著改善肝脏氧化应激损伤,治疗效果优于西药。

Objective To observe the effect of acupuncture on oxidative stress in the liver of rats modeled for Hashimoto's thyroiditis.Methods Forty healthy female rats were randomly divided into blank group,model group,western medicine group and acupuncture group,10 in each group.In addition to the blank group,the other three groups were prepared as Hashimoto's thyroiditis models,and from the 9th week after successful modeling,the model group and the blank group were fed normally,the western medicine group was treated with selenium yeast gavage,and the acupuncture group was treated with acupuncture.In the 14th week,serum TSH,FT3,FT4,TG-Ab,TPO-Ab and ALT were detected by ELISA;MDA,SOD and CAT were measured by biochemical method,and liver tissue was observed by HE staining.Results Compared with the blank group,serum TSH decreased in the model group(P<0.05),and the levels of FT3,FT4,TG-Ab,TPO-Ab,ALT,and MDA,SOD,and CAT increased in the model group(P<0.05);compared with the model group,serum TSH increased significantly in the acupuncture group and the western medicine group(P<0.05),and the levels of FT3,FT4,TG-Ab,TPO-Ab,ALT and MDA,SOD,CAT levels were significantly lower(P<0.05);compared with the western medicine group,rats in the acupuncture group had higher serum TSH(P<0.05)and lower FT3,FT4,TG-Ab,TPO-Ab,ALT and MDA,SOD,CAT levels(P<0.05).Compared with the blank group,there was obvious stasis in the liver tissue of the model group;compared with the model group,the liver stasis was improved in both the acupuncture group and the western medicine group,and the acupuncture group showed better improvement than the western medicine group.Conclusion Acupuncture can significantly improve liver oxidative stress injury,and the therapeutic effect is better than western medicine.