孕晚期妊娠糖尿病患者血清lncRNA FGD5-AS1、miR-103a-3p表达与产褥期感染的相关性研究

Correlation between serum lncRNA FGD5-AS1,miR-103a-3p and puerperal infectionin patients with gestational diabetes mellitus in late pregnancy

目的探讨孕晚期妊娠糖尿病(GDM)患者血清长链非编码RNA FGD5-AS1(lncRNA FGD5-AS1)、微小RNA-103a-3p(miR-103a-3p)与产褥期感染(PI)的相关性。方法回顾性选取2022年1月至2023年6月在该院就诊并住院分娩的168例孕晚期GDM患者作为试验组,并根据是否发生PI将患者分为感染组(96例)和未感染组(72例),同时选取同期在该院产检且血糖正常的120例孕晚期女性作为对照组。实时荧光定量PCR(qRT-PCR)检测lncRNA FGD5-AS1与miR-103a-3p表达水平,多因素Logistic回归分析孕晚期GDM患者发生PI的影响因素,StarBase网站分析lncRNA FGD5-AS1与miR-103a-3p的关系,Pearson相关分析lncRNA FGD5-AS1与miR-103a-3p的相关性,受试者工作特征(ROC)曲线评估lncRNA FGD5-AS1及miR-103a-3p预测PI发生的价值。结果试验组和对照组血清lncRNA FGD5-AS1与miR-103a-3p表达水平比较差异有统计学意义(P<0.05),感染组血清lncRNA FGD5-AS1表达水平显著高于未感染组(P<0.05),但感染组血清miR-103a-3p表达水平显著低于未感染组(P<0.05)。lncRNA FGD5-AS1表达水平是孕晚期GDM患者发生PI的独立危险因素(P<0.05),miR-103a-3p表达水平是孕晚期GDM患者发生PI的独立保护因素(P<0.05)。lncRNA FGD5-AS1与miR-103a-3p表达水平呈负相关(r=-0.409,P<0.001)。lncRNA FGD5-AS1与miR-103a-3p二者联合预测孕晚期GDM患者发生PI的效能优于血清lncRNA FGD5-AS1及miR-103a-3p单项预测(P<0.05)。结论lncRNA FGD5-AS1是孕晚期GDM患者发生PI的独立危险因素,而miR-103a-3p是孕晚期GDM患者发生PI的独立保护因素;二者联合检测预测孕晚期GDM患者发生PI的价值更高。

Objective To investigate the correlation between serum long non-coding RNA FGD5-AS1(lncRNA FGD5-AS1),microRNA-103a-3p(miR-103a-3p)and puerperal infection(PI)in patients with gestational diabetes mellitus(GDM)in late pregnancy.Methods A total of 168 late pregnancy GDM patients who were hospitalized and delivered in the hospital from January 2022 to June 2023 were retrospectively selected as the experimental group,and the patients were separated into an infected group(96 cases)and an uninfected group(72 cases)based on whether they had PI.At the same time,120 late pregnant women who underwent prenatal examination in the hospital and had normal gestational blood glucose were selected as the control group.Real-time fluorescence quantitative PCR(qRT-PCR)was applied to detect the expression levels of lncRNA FGD5-AS1 and miR-103a-3p.Multivariate Logistic regression was applied to analyze the influencing factors of PI in late pregnancy GDM patients.StarBase website was applied to analyze the relationship between lncRNA FGD5-AS1 and miR-103a-3p.Pearson was applied to analyze the correlation between lncRNA FGD5-AS1 and miR-103a-3p.Receiver operating characteristic(ROC)curve was applied to evaluate the value of lncRNA FGD5-AS1 and miR-103a-3p in predicting the occurrence of PI.Results There was a statistically significant difference in the expression levels of serum lncRNA FGD5-AS1 and miR-103a-3p between the experimental group and the control group(P<0.05),the expression level of serum lncRNA FGD5-AS1 in the infected group was obviously higher than that in the uninfected group(P<0.05),but the expression level of serum miR-103a-3p in the infected group was obviously lower than that in the uninfected group(P<0.05).The expression level of lncRNA FGD5-AS1 was an independent risk factor for PI in late-pregnancy GDM patients(P<0.05),and the expression level of miR-103a-3p was an independent protective factor for PI in late-pregnancy GDM patients(P<0.05).There was a negative correlation between lncRNA FGD5-AS1 and miR-103a-3p expression level(r=-0.409,P<0.001).The efficacy of the combined detection of lncRNA FGD5-AS1 and miR-103a-3p for predicting PI in late pregnancy GDM patients was superior to that of serum lncRNA FGD5-AS1 and miR-103a-3p alone(P<0.05).Conclusion LncRNA FGD5-AS1 is an independent risk factor for PI in late pregnancy GDM patients,while miR-103a-3p is an independent protective factor for PI in late pregnancy GDM patients.The combined detection has higher value for predicting PI in late pregnancy GDM patients.