pH荧光分子探针与人血清白蛋白之间相互作用的研究

Study on Interaction between the pH Fluorescent Molecule Probe and Human Serum Albumin

在模拟生理条件下,用光谱法研究了pH荧光探针WZK-33与人血清白蛋白(HSA)的相互作用机制。由光谱谱图结果可以得出,此探针可以猝灭HSA的荧光,探针与HSA之间的猝灭机制主要是静态猝灭方式。由热力学数据确定了二者之间的作用力类型为范德华力和氢键,结合位点为一个。二者之间的结合距离为4.77 nm。同步荧光、三维荧光光谱以及CD光谱表明探针改变了HSA的构象。

The binding of pH fluorescent probe(WZK-33)with human serum albumin(HSA)in physiological buffer(pH=7.40)was investigated by the multispectroscopic technique.Spectroscopic analysis revealed that the probe could quench the fluorescence of HSA and the quenching mechanism between probe WZK-33 and HSA was static quenching.The calculated thermodynamic parameters revealed that the binding of WZK-33-HSA was relied on Van der Waals forces and hydrogen bond and the number of binding site was to be one.The binding distance of WZK-33 with HSA was calculated to be 4.77 nm.The results of synchronous fluorescence spectra,three-dimensional fluorescence spectra and CD spectra demonstrated that the conformation of HSA was changed by the binding of WZK-33.