微柱凝胶技术在ABO血型不合新生儿溶血病诊断及输血前检验中的应用价值

Application value of micro column gel technology in the diagnosis of ABO incompatible hemolytic disease of newborn and pre-transfusion test

目的分析微柱凝胶技术(MCGT)在ABO血型不合新生儿溶血病(HDN)诊断以及输血前检验中的应用价值。方法选取2021年1月至2022年12月收治的200例ABO-HDN患儿为研究对象,另随机选取同期ABO同型血红细胞制剂血样本5份进行主侧交叉配血试验。采用MCGT与凝聚胺技术(MPT)进行交叉配血。比较两种技术的检测结果。结果交叉配血共进行1000次,MCGT的血清、放散液配血不合为740、575次;MPT的血清、放散液配血不合为40、55次。MCGT血清、放散液交叉配血不合率高于MPT,差异具有统计学意义(P<0.05)。MCGT的灵敏度、特异度及准确度高于MPT,差异具有统计学意义(P<0.05)。结论在ABO-HDN诊断及输血前检验中,MCGT的血液检测不合率较高,且灵敏度、特异度及准确度高于MPT。

Objective To analyze the application value of micro column gel technology(MCGT)in the diagnosis of ABO hemolytic disease of newborn(HDN)and pre-transfusion test.Methods A total of 200 children with ABO-HDN admitted from January 2021 to December 2022 were selected as the research objects.In addition,5 blood samples of ABO homotypic red blood cell preparations in the same period were randomly selected for the main-side cross-matching test.Cross matching of blood was carried out by MCGT and manual polybrene test(MPT).The test results of the two techniques were compared.Results A total of 1000 times of cross matching were performed,and 740 and 575 times of serum and release liquid of MCGT were not matched;40 and 55 times of serum and release liquid of MPT were not matched.The cross-matching incompatibility rate of MCGT serum and release liquid were higher than those of MPT,and the differences were statistically significant(P<0.05).The sensitivity,specificity and accuracy of MCGT were higher than those of MPT,and the differences were statistically significant(P<0.05).Conclusion In ABO-HDN diagnosis and pre-transfusion test,the blood test incompatibility rate of MCGT is higher,and the sensitivity,specificity and accuracy are higher than those of MPT.