补体相关蛋白在HIV/AIDS湿热内蕴证患者中的差异性表达

Disease syndrome correlation between HIV/AIDS patients and HIV/AIDS patients with damp-heat intrinsic syndrome based on complement system

目的 探讨补体系统相关因子在HIV/AIDS患者中及其与HIV/AIDS湿热内蕴证侯的表达特征。方法共纳入2023年3-5月于广西壮族自治区某地区就诊的HIV/AIDS患者(简称HIV组),其中包括HIV/AIDS湿热内蕴证患者组(简称HIVsr组)与HIV/AIDS无证可辨患者组(简称HIVwz组)及同期在门诊进行体检的健康对照组。收集其基本信息并采集抗凝血液,应用流式细胞术检测T细胞亚群数量,酶联免疫吸附试验检测补体相关因子血浆表达水平,对数据进行统计分析。结果 共纳入HIV组45例,其中包括HIVsr组30例与HIVwz组15例,与健康对照组15例。免疫指标对比中,HIVsr组与健康对照组相比TH/TS表达下降(P<0.05)。通路相关因子血浆表达水平对比中,HIV组与健康对照组相比除C3、C5aR、C5b因子外,C1q、MASP2、MBL等12个因子表达均下降(P<0.05),HIVsr组与HIVwz组相比C1q、C5aR、IL-6、TNF-α表达显著降低(P<0.05);HIVsr组因子C3aR与湿热内蕴证证候学量表积分呈负相关(r=-0.613,P<0.05),多元线性回归分析显示C3aR是量表积分的影响因素。结论 补体系统中经典及凝集素通路参与HIV/AIDS患者病理过程,且HIV/AIDS湿热内蕴证患者的证候生物学基础与经典通路相关。C3aR对临床诊断HIV/AIDS湿热内蕴证患者有一定鉴别价值。

Objective To investigate the expression characteristics of complement system related factors in HIV/AIDS patients and their association with the damp-heat intrinsic syndrome of HIV/AIDS.Methods HIV/AIDS patients(HIV group)who were admitted to a hospital in Guangxi Zhuang Autonomous Region from March to May 2023 were included,including the HIV/AIDS patients with damp-heat intrinsic syndrome group(HIVsr group),the HIV/AIDS patients without identifiable syndromes group(HIVwz group),and the healthy control group who underwent outpatient physical examinations.Baseline information and anticoagulant blood samples were collected.The number of T cell subsets was detected by flow cytometry,and the plasma expression levels of complement-related factors were determined by enzyme-linked immunosorbent assay(ELISA).Data were statistically analyzed.Results A totally of 45 cases were included in the HIV group,with 30 cases in the HIVsr group,15 cases in the HIVwz group,and 15 cases in the healthy control group.The immunological indicator of helper T cell/suppressor T cell(TH/TS)expression showed a decrease in the HIVsr group compared to the healthy control group(P<0.05).Compared with the healthy control group,the expression levels of 12 factors including C1q,MASP2 and MBL were decreased in HIV group(P<0.05),except for factors C3,C5aR,and C5b.The HIVsr group showed significantly lower expression of C1q,C5aR,IL-6,and TNF-αcompared to the HIVwz group(P<0.05).C3aR in the HIVsr group was negatively correlated with the score of Damp-Heat Intrinsic Syndrome(r=-0.613,P<0.05).Multiple linear regression analysis indicated that C3aR was an influencing factor for the scale score.Conclusions Classical and lectin pathways in the complement system are related to the pathological process of HIV/AIDS,and the biological basis of the damp-heat intrinsic syndrome in HIV/AIDS patients is associated with the classical pathway.C3aR has some differential diagnostic value for HIV/AIDS patients with Damp-Heat Intrinsic Syndrome.