子宫内膜息肉患者TRPC3和TRPC6蛋白表达的临床意义及与孕酮抵抗的相关性研究

Clinical significance of transient receptor potential-canonical chanmel 3 and transient receptor potential-canonical channel 6 protein expression and correlation with progesterone resistance in patients with endometrial polyps

目的探究子宫内膜息肉中瞬时受体电位通道C亚族3(TRPC3)、瞬时受体电位通道C亚族6(TRPC6)蛋白表达与孕酮抵抗的相关性。方法选取石家庄市第六医院2021年2月至2023年2月期间收治的65例子宫内膜息肉患者作为子宫内膜息肉组,于同期同一医院选取65例因不孕症进行宫腔镜检查的患者作为正常子宫内膜对照组。采用免疫组化法检测正常子宫内膜组织和子宫内膜息肉组织中TRPC3、TRPC6、孕酮受体(PR)蛋白表达情况;采用Spearman等级相关分析探究子宫内膜息肉组织中TRPC3、TRPC6与PR的相关性;采用Logistic回归分析子宫内膜息肉发生的影响因素。结果子宫内膜息肉组和正常子宫内膜对照组慢性子宫内膜炎、肥胖所占比例比较,差异具有统计学意义(P<0.05);与正常子宫内膜组织相比,子宫内膜息肉组织中TRPC3蛋白(56.92%vs.84.46%,χ^(2)=12.048,P=0.001)、TRPC6蛋白(53.85%vs.83.08%,χ^(2)=12.861,P<0.001)阳性表达率升高,PR蛋白(86.15%vs.46.15%,χ^(2)=23.224,P<0.001)阳性表达率降低。Spearman等级相关性分析显示,子宫内膜息肉组织中TRPC3、TRPC6与PR表达呈负相关(r=-0.289、-0.323,P<0.05);TRPC3、TRPC6、慢性子宫内膜炎、肥胖是影响子宫内膜息肉发生的相关因素(P<0.05)。结论子宫内膜息肉组织中TRPC3、TRPC6与PR表达呈负相关,且是发生子宫内膜息肉的影响因素。

Objective To investigate the correlation between transient receptor potential-canonical channel 3(TRPC3)and transient receptor potential-canonical channel 6(TRPC6)protein expressions and progesterone resistance in endometrial polyps.Methods A total of 65 patients with endometrial polyps admitted to the Sixth Hospital of Shijiazhuang from February 2021 to February 2023 were collected as the endometrial polyp group,65 patients who underwent hysteroscopy due to infertility in the same hospital during the same period were regarded as the normal endometrial control group.Immunohistochemical methods were applied to detect the expression of TRPC3,TRPC6,and progesterone receptor(PR)proteins in endometrial polyp tissue and normal endometrial tissue;Spearman rank correlation was applied to analyze the correlation between TRPC3,TRPC6,and PR in endometrial polyp tissue;Logistic regression was applied to analyze the relevant influencing factors for the occurrence of endometrial polyps.Results The proportion of chronic endometritis and obesity between endometrial polyp group and normal endometrial control group had statistically significant differences(P<0.05);compared with normal endometrial tissue,the positive expression rates of TRPC3 protein(56.92%vs.84.46%,χ^(2)=12.048,P=0.001)and TRPC6 protein(53.85%vs.83.08%,χ^(2)=12.861,P<0.001)in endometrial polyp tissue increased,while the positive expression rate of PR protein(86.15%vs.46.15%,χ^(2)=23.224,P<0.001)decreased.Spearman rank correlation analysis showed a negative correlation between TRPC3,TRPC6,and PR expression in endometrial polyp tissue(r=-0.289,-0.323,P<0.05);TRPC3,TRPC6,chronic endometritis,and obesity were the influencing factors for the occurrence of endometrial polyps(P<0.05).Conclusions The expressions of TRPC3 and TRPC6 in endometrial polyp tissue are negatively correlated with PR and are influencing factors for the occurrence of endometrial polyps.