摘要
江苏省某猪场疑似发生猪流行性腹泻(Porcine epidemic diarrhea,PED),采集病料后,经RT-PCR检测PEDV呈阳性。病毒感染Vero细胞能够引起明显的细胞病变,间接免疫荧光鉴定结果显示病毒感染细胞后能被抗PEDV N蛋白的特异性单克隆抗体识别。通过全基因组序列分析,鉴定该毒株为PEDV G2b亚型变异株,并将其命名为PEDV JS-B株。
PED was suspected to have occurred in a pig farm in Jiangsu Province,and after collection of disease material,PEDV was tested positive by RT-PCR.Virus-infected Vero cells were able to cause significant cytopathic changes,and the immunofluorescence results showed that the virus-infected cells could be recognized by a specific monoclonal antibody against PEDV N protein.Through whole genome sequence analysis,this strain was identified as a PEDV G2b subtype variant and designated PEDV JS-B strain.
作者
刘曦
贾相超
李晨曦
李自力
LIU Xi;JIA Xiang-chao;LI Chen-xi;LI Zi-li(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Preventive Veterinary Medicine in Hubei Province,Wuhan 430070,China;State Key Laboratory of Agricultural Microbiology,Wuhan 430070,China)
出处
《湖北农业科学》
2024年第7期134-140,共7页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(32072845)。
关键词
猪流行性腹泻病毒
分离鉴定
变异毒株
分段测序
序列分析
porcine epidemic diarrhea virus
isolation and identification
variant strain
segmental sequencing
sequence analysis
