基于电子鼻技术的烟丝受虫害前后挥发性成分差异分析

Analysis of differences in volatile components of cut tobacco before and after pest infestation based on electronic nose technology

针对烟草(Nicotiana tabacum L.)中烟草甲(Lasioderma serricorne)检测技术不完善,检测结果不可靠等问题,以电子鼻检测结果为基础,通过主成分分析法(PCA)和Loading分析法对数据结果进行分析。结果表明,4种烟丝的主要挥发物质为硫类物质、短链烷烃、醇醚醛酮、小分子氮氧化合物等,虫样及性激素的主要挥发性物质是小分子氮氧化合物、硫化物。受虫害后烟丝中硫类化合物明显降低,在对无机硫化物敏感的传感器(W1W)中浓香虫害烟丝的响应值比浓香烟丝下降48.33%,清香虫害烟丝比清香烟丝下降59.48%;在对有机硫化物敏感的传感器(W2W)中浓香虫害烟丝的响应值比浓香烟丝下降35.72%,清香虫害烟丝比清香烟丝下降48.55%。PCA结果显示,4种烟丝样品表现出非常好的区分度,雌虫、雄虫样品在占比高达99.70%的主成分1上几乎没有差别。Loading分析结果显示,烟丝样品中气味主要成分为硫类物质,烟草甲虫样气味主要成分是小分子氮氧化物,其次为硫类物质。

In response to the problems of incomplete detection technology and unreliable detection results for Lasioderma serricorne in Nicotiana tabacum L.,based on the results of electronic nose detection,the data results were analyzed using principal component anal-ysis(PCA)and loading analysis.The results showed that the main volatile substances of the four cut tobacco were sulfur compounds,short-chain alkanes,alcohol ether aldehydes and ketones,small molecule nitrogen oxides,etc.The main volatile substances of insect like and sex hormones were small molecule nitrogen oxides and sulfides.After being damaged by insects,the sulfur compounds in cut tobacco decreased significantly.In the sensor(W1W)sensitive to inorganic sulfides,the response value of strong aroma insect cut to-bacco decreased by 48.33%compared to strong aroma cut tobacco,and light aroma insect cut tobacco decreased by 59.48%compared to light aroma cut tobacco;in the sensor(W2W)sensitive to organic sulfides,the response value of the strong aroma pest cut tobacco decreased by 35.72%compared to the strong aroma cut tobacco,and the light aroma pest cut tobacco decreased by 48.55%compared to the light aroma cut tobacco.The PCA results showed that the four cut tobacco samples exhibited excellent discrimination,with al-most no difference in principal component 1,which accounted for 99.70%,between female and male samples.The loading analysis re-sults showed that the main odor component in the cut tobacco sample was sulfur compounds,while the main odor component in the to-bacco beetle sample was small molecule nitrogen oxides,followed by sulfur compounds.