秸秆与生物炭施加对茉莉园土壤真菌群落及有机碳库特征的影响

Effects of Straw and Biochar Amendments on Characteristics of Soil Fungal Community and Organic Carbon Pool in Jasminum sambac Garden

为阐明秸秆与生物炭施加后茉莉园土壤真菌群落及有机碳库组分分配的变化特征,测定分析了茉莉园对照、秸秆和生物炭处理0~15 cm土壤真菌群落特征与有机碳库组分,并计算其碳库管理指数(CPMI).结果表明,秸秆与生物炭施加后土壤真菌群落多样性均降低,各处理中优势菌属结构发生变化,生物炭处理下土壤真菌群落结构与秸秆处理和对照组差异显著.冗余分析(RDA)表明,土壤真菌群落结构主要受土壤容重、C∶N、盐度和TN影响.另外,秸秆处理下土壤活性有机碳(LOC)与对照组相比显著增加87.44%(P<0.05),生物炭处理中土壤可溶性有机碳(DOC)、微生物生物量碳(MBC)较对照组分别显著增加22.27%和23.17%(P<0.05).相比于对照组,秸秆处理下碳库活度(L)显著提高(P<0.05),生物炭处理中碳库指数(CPI)显著增加(P<0.05).Spearman相关性分析表明土壤活性有机碳组分受到真菌优势菌属的调控. FUNGuild功能预测结果表明,秸秆与生物炭施加后腐生营养型及其兼性营养型真菌对土壤活性有机碳组分和碳库管理指数有着重要影响.

In order to elucidate the changes in the soil fungal community and soil organic carbon components of a Jasminum sambac garden after straw and biochar application,we measured the organic carbon components and soil fungal community of the 0-15 cm soil layer in a J.sambac garden,which was divided into a control group,straw treatment group,and biochar treatment group.The carbon pool management index(CPMI)was also calculated.The results showed that the diversity of the soil fungal community was decreased after straw and biochar application,and the structure of dominant fungal genera was changed in each treatment.The soil fungal community structure in the biochar treatment was significantly different from that in the straw treatment and control groups.Redundancy analysis(RDA)showed that soil fungal community structure was mainly affected by soil bulk density,C∶N,salinity,and TN.Secondly,compared with that in the control group,soil labile organic carbon(LOC)in the straw treatment group was significantly increased by 87.44%(P<0.05),whereas soil dissolved organic carbon(DOC)and microbial biomass carbon(MBC)in the biochar treatment group were significantly increased by 22.27%and 23.17%(P<0.05),respectively.Further,compared with that in the control group,the carbon pool activity(L)under straw treatment was significantly increased(P<0.05),and the carbon pool index(CPI)under biochar treatment was significantly increased(P<0.05).Spearman correlation analysis showed that the distribution characteristics of soil organic carbon active components were regulated by the dominant fungi.FUNGuild functional prediction results showed that saprophytic and its facultative nutritional fungi had an important impact on soil organic carbon active components and carbon pool management index after straw and biochar application.