红景天对急性脑出血后神经功能影响及作用机制研究

Study on the Effect and Mechanism of Rhodiola Rosea on Neurological Function after Acute Cerebral Hemorrhage

目的:观察红景天对急性脑出血(acute cerebral hemorrhage,ACH)大鼠神经功能影响并探讨其作用机制。方法:将实验大鼠随机分为假手术组(Sham组)、急性脑出血组(ACH组)和红景天组。分别对各组大鼠进行神经功能Bederson评分及行为学评估,透射电镜(TEM)观察细胞超微结构,ELISA检测白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和转化生长因子-β(TGF-β)表达,免疫荧光法检测CD16、CD206表达,并对各组谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、铁(Fe)含量进行测定。结果:经红景天治疗后ACH大鼠的神经功能缺损评分明显降低,饮水、摄食、活动等行为学评估改善明显,死亡率下降,差异有统计学意义(P<0.05);经TEM观察发现,ACH组血肿周围组织疏松,核膜结构不清晰,细胞器减少,线粒体肿胀,数目减少,并出现空泡样改变。经红景天治疗后,细胞肿胀减轻,结构保持较完整。造模后,ACH组的GSH-PX表达下降,MDA表达升高,Fe含量升高,经红景天治疗后,GSH-PX升高,MDA、Fe含量下降;ELISA结果显示,与Sham组比较,ACH组中IL-1β、TNF-α表达明显升高(P<0.05);与ACH组比较,红景天组显著下调IL-1β、TNF-α表达,上调TGF-β表达(P<0.05);ACH组CD16荧光表达高于红景天组,CD206低于红景天组。结论:红景天对急性脑出血有较好的治疗作用,其机制可能与抑制炎症反应、降低氧化应激、减轻铁死亡及促进小胶质细胞极化有关。

Objective:To observe the effect of Rhodiola rosea on the neurological function of rats with acute cerebral hemorrhage(ACH)and to explore its mechanism of action.Methods:The experimental rats were randomly divided into sham operation group(Sham group),acute cerebral hemorrhage group(ACH group)and Rhodiola rosea group.The Bederson score of neurological function and behavioral evaluation were performed on the rats in each group,and the cell ultra-structure was observed by transmission electron microscopy(TEM).The expression of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and transforming growth factor-β(TGF-β)was detected by ELISA,and the expression of CD16 and CD206 was detected by immunofluorescence.The contents of glutathione peroxidase(GSH-PX),malondialdehyde(MDA)and iron(Fe)in each group were determined.Results:After treatment with Rhodiola rosea,the neurological deficit score of ACH rats was significantly reduced,and the behavioral evaluations such as drinking,eating,and activity were significantly improved,and the mortality rate decreased,with statistically significant differences(P<0.05).TEM observation showed that the tissue around the hematoma in the ACH group was loose,the nuclear membrane structure was unclear,the organelles were reduced,the mitochondria were swollen,the number was reduced,and vacuolar changes occurred.After treatment with Rhodiola rosea,the cell swelling was reduced and the structure remained relatively intact.After modeling,the expression of GSH-PX in the ACH group decreased,the expression of MDA increased,and the content of Fe increased.After treatment with Rhodiola rosea,the expression of GSH-PX increased,and the contents of MDA and Fe decreased.ELISA results showed that compared with the Sham group,the expression of IL-1βand TNF-αin the ACH group was significantly increased(P<0.05);compared with the ACH group,the Rhodiola rosea group significantly downregulated the expression of IL-1βand TNF-α,and up-regulated the expression of TGF-β(P<0.05);the CD16 fluorescence expression in the ACH group was higher than that in the Rhodiola rosea group,and the CD206 was lower than that in the Rhodiola rosea group.Conclusion:Rhodiola rosea has a good therapeutic effect on acute cerebral hemorrhage,and its mechanism may be related to inhibiting inflammatory response,reducing oxidative stress,alleviating ferroptosis and promoting micro-glial polarization.