推中脘联合参香散贴敷通过SCF/C-kit信号通路对胃食管反流的作用研究

Study on the Effect of Zhongwan Acupoint Combined with Shenxiangsan Application on Gastroesophageal Reflux Via SCF/C-kit Signaling Pathway

目的:探讨推中脘联合参香散贴敷通过干细胞因子(stem cell factor,SCF)/C-kit信号通路对胃食管反流(Gastroesophageal reflux,GER)的调控作用。方法:通过饮食不节法和导尿管球囊扩张法构建GER动物模型,分别用推中脘、参香散贴敷以及推中脘联合参香散贴敷治疗GER模型兔子。苏木精-伊红染色观察胃窦组织病理损伤。免疫荧光检测C-kit荧光强度。RT-qPCR检测SCF和C-kit mRNA相对表达。Western blot检测SCF、C-kit和连接蛋白43(Connexin 43,CX43)蛋白表达。结果:GER组兔子胃窦组织中细胞炎性浸润明显、胃黏膜上皮细胞增生,且组织中SCF、C-kit和CX43表达明显降低。经推中脘和参香散贴敷治疗可降低胃窦组织炎性浸润程度,促进SCF、C-kit和CX43表达。而与推中脘或参香散贴敷单独治疗组比较,推中脘联合参香散贴敷治疗组中细胞炎性浸润较单独治疗组减弱,几乎没有细胞增生,SCF、C-kit和CX43表达明显升高。结论:推中脘联合参香散贴敷治疗可显著改善GER,这可能通过调控SCF/C-kit信号通路实现。

Objective:To investigate the regulatory effect of Tuizhongwan combined with Shenxiang powder application on gastroesophageal reflux(GER)through stem cell factor(SCF)/C-kit signaling pathway.Methods:GER animal models were established by dietary indiscriminateness and catheter balloon dilatation.GER rabbits were treated with Tuizhongwan,Shenxiang powder and Tuizhongwan combined with Shenxiang powder application,respectively.Pathological damage of gastric antrum tissue was observed by hematoxylin-eosin staining.Immunofluorescence was used to detect the fluorescence intensity of C-kit.RT-qPCR was used to detect the relative expression of SCF and Ckit mRNA.Western blot was used to detect the protein expression of SCF,C-kit and connexin 43(CX43).Results:In the GER group,cellular inflammatory infiltration was obvious in the gastric antrum tissue of rabbits,gastric mucosal epithelial cells proliferated,and the expression of SCF,C-kit and CX43 in the tissue was significantly reduced.Tuizhongwan and Shenxiang powder application can reduce the degree of inflammatory infiltration in gastric antrum tissue and promote the expression of SCF,C-kit and CX43.Compared with the Tuizhongwan or Shenxiang powder application alone group,the Tuizhongwan combined with Shenxiangsan application group showed a weakened cellular inflammatory infiltration, almost no cell proliferation, and significantly increased SCF, C-kit and CX43 expression. Conclusion:Tuizhongwan combined with Shenxiang powder application can significantly improve GER, which may be achieved byregulating the SCF/C-kit signaling pathway.