翘芩清肺剂对咳嗽性哮喘的作用机制研究

Study on the Mechanism of Qiaoqin QingfeiFormula in Treating Cough Asthma

目的:探讨翘芩清肺剂对咳嗽变异性哮喘(CVA)的防治机制。方法:SD大鼠36只随机分为正常组、模型组、布地奈德组、翘芩清肺剂高、中、低剂量组等6组。采用卵清白蛋白(OVA)方法建立大鼠CVA模型。从第2天开始灌胃给药,每日1次共30d。实验完毕取大鼠右上叶肺组织HE染色;取全血、肺泡灌洗液(broncho-alveolar lavage fluid, BALF)做白细胞分类计数;ELISA法检测血清IL-6、TNF-α、NGF、SP含量。结果:与空白组相比,模型组肺血管壁和气管壁周围大量炎性细胞浸润;全血和BALF嗜酸性粒细胞百分比%、IL-6、TNF-α、NGF、SP显著升高(P<0.05),有统计学意义。与模型组相比,翘芩清肺剂高、中剂量可改善肺组织炎症细胞浸润;翘芩清肺剂中、低量组明显降低全血及BALF嗜酸性粒细胞百分比%、IL-6、TNF-α、NGF、SP水平(P<0.05),有统计学意义。结论:翘芩清肺剂可有效抑制神经多肽分子NGF、SP,调控气道神经源性炎症,改善CVA气道高反。

Objective:To explore the preventive and therapeutic mechanism of Qiaoqin Qingfei Formula on cough variant asthma(CVA).Method:36SD rats were randomly divided into 6groups:normal group,model group,budesonide group,high,medium,and low dose groups of Qiaoqin Qingfei Formula.Establishing a rat CVA model using ovalbumin(OVA)method.Starting from the second day,the medication was administered by gavage once a day for a total of 30days.After the experiment,HE staining was performed on the right upper lobe lung tissue of rats;Take whole blood and bronchoalveolar lavage fluid(BALF)for white blood cell classification and counting;ELISA method was used to detect the levels of serum IL-6,TNF-α,NGF,and SP.Result:Compared with the blank group,a large number of inflammatory cells infiltrated around the pulmonary vascular wall and tracheal wall in the model group;The percentages of eosinophils in whole blood and BALF,as well as the levels of IL-6,TNF-α,NGF,and SP were significantly elevated(P<0.05),with statistical significance.Compared with the model group,high and medium doses of Qiaoqin Qingfei Formula can improve the infiltration of inflammatory cells in lung tissue.The percentage of eosinophils in whole blood and BALF and the levels of IL-6,TNF-α,NGF and SP were significantly decreased in the middle and low dose Qiaoqin Qingfei group(P<0.05).Clusion:Qiaoqin Qingfei Formula can effectively inhibit neuropeptide molecules NGF and SP,regulate airway neurogenic inflammation,and improve CVA airway hyperreflexes.