姜黄素类物质微波辅助提取的工艺优化及其抗氧化活性研究

Optimization of microwave-assisted extraction process for curcuminoid-like substances and their antioxidant activity

采用微波辅助提取技术,考察了提取时料液比、乙醇浓度、微波功率、提取时间对姜黄素类物质的含量及抗氧化活性的影响。结果表明,料液比为1∶25、乙醇浓度80%、微波功率40 W、微波时间50 s时,姜黄素含量为3.435 mg/g,双去甲氧基姜黄素为0.304 mg/g,去甲氧基姜黄素含量为0.450 mg/g,提取液对DPPH清除率达到59.16%,对羟基自由基清除率达到86.82%。

Microwave-assisted extraction technology was employed to explore the effects of solid-to-liquid ratio,ethanol concentration,microwave power,and extraction time on the yield and antioxidant activity of curcuminoidlike substances.The optimal conditions were found as solid-to-liquid ratio of 1∶25,ethanol concentration of 80%,microwave power of 40 W,and an extraction time of 50 seconds.Under these conditions,the yields of curcumin,bisdemethoxycurcumin,and demethoxycurcumin were 3.435,0.304,and 0.450 mg/g,respectively.The extraction solution exhibited a DPPH radical scavenging rate of 59.16% and a hydroxyl radical scavenging rate of 86.82%.