基于细胞焦亡探讨牡荆素对小鼠肝缺血再灌注损伤的保护作用

Protective effect of Vitexin on hepatic ischemia-reperfusion injury in mice base on pyroptosis

目的:探讨牡荆素抑制细胞焦亡对小鼠肝缺血再灌注损伤的保护作用。方法:采用C57小鼠建立肝脏缺血再灌注损伤模型,实验动物分为假手术组、模型组及牡荆素组;检测小鼠血清ALT、AST及IL-1β含量,qRT-PCR检测IL-1β与GSDMD mRNA的表达,western blot检测各组小鼠肝组织Caspase-1与GSDMD的表达。结果:与假手术组相比,模型组血清中ALT、AST与IL-1β水平明显升高;而与模型组相比较,牡荆素组ALT、AST与IL-1β水平明显降低(P<0.05)。同时,牡荆素可减少缺血再灌注诱导肝脏Caspase-1与GSDMD蛋白表达(P<0.05)。结论:牡荆素对小鼠肝缺血再灌注损伤具有明显的改善作用,其作用可能与抑制细胞焦亡有关。

Objective:To investigate the protective effect of Vitexin on hepatic ischemia-reperfusion injury in mice base on pyroptosis.Methods:C57 mice were divided into sham operation group,model group and Vitexin group.The levels of alt,AST and IL-1βin serum were detected.The expressions of IL-1βand GSDMD mRNA were detected by QRT PCR.The expressions of Caspase 1 and GSDMD in liver tissue were detected by Western blot.Results:compared with the sham operation group,the levels of ALT,AST and IL-1βin model group were significantly higher than those in model group,while the levels of alt,AST and IL-1βin Vitexin group were significantly lower than those in model group(P<0.05).At the same time,Vitexin could reduce the expression of Caspasel and GSDMD protein induced by ischemia-reperfusion(P<0.05).Conclusion:Vitexin can significantly improve liver ischemia reperfusion injury in mice,which may be related to the inhibition of pyrocytosis.