结核分枝杆菌免疫逃逸相关蛋白Mce2D的生物信息学分析

Bioinformatics analysis of immune escape related protein Mce2D in Mycobacterium tuberculosis

目的 应用生物信息学方法分析预测结核分枝杆菌Rv0592基因编码蛋白Mce2D的结构和功能。方法 从NCBI网站查询Rv0592基因及其编码蛋白的基本信息,通过Uniprot数据库获取Mce2D蛋白的氨基酸序列。运用ORF Finder、Protpatam、SOPMA、SWISS-MODEL、ProtScale、Signal IP 4.1 Server、TMHMM、ProtComp、SYFPEITHI、IEDB等生物信息学软件分别对Mce2D蛋白的开放阅读框架、理化性质、二级结构、三级结构、亲疏水性、信号肽、跨膜区、亚细胞定位、T细胞表位和B细胞表位等生物特征进行预测。结果 Rv0592基因全长1 527 bp,共有8个开放阅读框架,编码蛋白的氨基酸数为508个。Mce2D蛋白的等电点为5.06,总平均亲水性为-0.109,为亲水蛋白。蛋白的二级结构中无规卷曲占42.52%、α-螺旋占41.54%,含有多个T细胞表位和B细胞表位。该蛋白有一个信号肽、跨膜螺旋数为一、亚细胞定位于细胞膜。结论 生物信息学预测Mce2D蛋白为含多个的B细胞和T细胞表位,可作为结核病诊断和治疗的有效靶点。

Objective To apply bioinformatics methods to analyze and predict the structure and function of the protein Mce2D encoded by Mycobacterium tuberculosis Rv0592 gene. Methods The basic information of the Rv0592 gene and its encoded protein was queried from the NCBI website, and the amino acid sequence of the Mce2D protein was obtained through the Uniprot database. Bioinformatics software such as ORF Finder, Protpatam, SOPMA,SWISS-MODEL,ProtScale, Signal IP 4.1 Server, TMHMM,ProtComp, SYFPEITHI,and IEDB were used to analyze the open reading frame, physicochemical properties, secondary structure, tertiary structure, hydrophilicity and hydrophilicity of Mce2D protein, respectively. structure, tertiary structure, hydrophilicity, signal peptide, transmembrane region, subcellular localization, T-cell epitope and B-cell epitope, and other biological features were predicted. Results The Rv0592 gene was 1 527 bp in length, with 8 open reading frames and 508 amino acids in the encoded protein.The isoelectric point of the Mce2D protein was 5.06,and the total average hydrophilicity was-0.109,making it a hydrophilic protein. The secondary structure of the protein is 42.52% randomly coiled and 41.54% alpha-helical, and contains multiple T-cell epitopes and B-cell epitopes. The protein has a signal peptide, a transmembrane helix number of one, and subcellular localization to the cell membrane. Conclusion Bioinformatics predicts that the Mce2D protein is a multiple-containing B-cell and T-cell epitope that may serve as an effective target for tuberculosis diagnosis and therapy.