以草莓花梗为外植体的高效组培快繁技术

An efficient tissue culture technique using strawberry peduncles as explants

健康种苗是草莓产业的重要组成部分,而组培快繁技术是生产健康种苗的重要一环。文章通过比较不同品种来源、不同器官来源外植体产生的不定芽发生率和再生芽数,来探究草莓花梗成苗的快繁技术。试验发现,带有花蕾和苞片部位的花梗能在接种后3周形成不定芽,6周形成丛生芽;不定芽发生率可达100%。这为草莓种质资源保存、健康种苗生产提供了有力保障。

Healthy seedling is important to strawberry industry,while tissue culture is an important role in producing it.This study explored a rapid propagation technology using strawberry peduncles as explants by comparing the rate of adventitious shoot and the number of regenerated shoots from different organs and cultivated varieties.It was found that peduncles with flower buds and bracts can form adventitious shoots and clustered seedlings in 3 weeks and 6 weeks respectively,and the adventitious shoot regeneration rate can reach 100%.It provides a strong guarantee for the preservation of strawberry germplasm resources and the production of healthy seedlings.