TDZ对白鹤芋增殖及白化效应的影响

Effect of TDZ on the proliferation and whitening effect of Spathiphyllum kochii

为建立高效优质的白鹤芋再生组培体系,该试验以白鹤芋不定芽为试验材料,分析其白化过程中的生理生化变化,探究其白化效应机理。采用单因素试验设计方法,研究噻苯隆(TDZ)对白鹤芋不定芽增殖及白化效应的影响,在培养5、10、15、20和25 d时随机抽取6瓶统计增殖有效芽(0.5 cm以上)和生根有效芽(2.0 cm以上)的不定芽数量,测定叶绿素含量、丙二醛含量、脯氨酸含量以及过氧化氢酶(CAT)活性。TDZ对白鹤芋不定芽增殖表现出高抑制低促进的效应,白化率随着TDZ浓度的增加而升高,0.05 mg·L^(-1)TDZ处理的总白化率显著低于其他处理,白鹤芋不定芽脯氨酸含量随TDZ处理时间延长逐渐升高,丙二醛含量先升高后降低,CAT活性随着处理时间的延长而逐渐降低。

In order to establish an efficient and high-quality regeneration tissue culture system of Spathiphyllum kochii,the experiment was conducted to analyze the physiological and biochemical changes in the process of indefinite germination and explore the albino of its whitening effect.The single factor experimental design method was used to study the effect of thidiazuron(TDZ)on the adventitious bud proliferation and albino effect of the Spathiphyllum kochii.On the 5,10,15,20 and 25 d of culture,6 bottles were randomly selected to count the number of adventitious buds with effective proliferation(more than 0.5 cm)and effective rooting(more than 2.0 cm).Chlorophyll content,malondialdehyde content,proline content and CAT activity were determined.TDZ showed high inhibition and low promotion effects on the proliferation of adventitious buds of Spathiphyllum kochii,and the whitening rate of Spathiphyllum kochii gradually increased with the increase of TDZ concentration.The total whitening rate of Spathiphyllum kochii with 0.05 mg·L^(-1) TDZ treatment was significantly lower than that of other treatments.Proline content of Spathiphyllum kochii increased gradually with TDZ treatment time,MDA content of Spathiphyllum kochii increased first and then decreased,and CAT activity of Spathiphyllum kochii decreased with TDZ treatment time.