溶无机磷假单胞菌鉴定及对黑麦草生长的影响

Screening of pseudomonas inorganic phosphate and its effect on ryegrass growth

【目的】研究假单胞菌属的溶解无机磷能力,以获得性状优良的菌株资源。【方法】将从植物根际分离的8株细菌接种于PKO培养基,采用钼锑钪比色法定量分析、分子生物学鉴定和半固体植物接种试验,探究了细菌溶无机磷能力和对黑麦草的促生作用,并对菌株进行初步鉴定。【结果】8株细菌在PKO液体培养基培养10 d后,溶解无机磷量在329.39~479.09μg/mL,其中菌株MPD4溶磷量最高;8株细菌均归属于假单胞菌属(Pseudomonas sp.)。【结论】与对照相比,8株细菌均可有效促进黑麦草的生长发育。

【Objective】Study the ability of Pseudomonas to dissolve inorganic phosphorus and obtain strain re⁃sources with excellent characters.【Method】Eight strains of bacteria isolated from plant rhizosphere were inoculated into PKO medium and quantitative analysis was conducted using molybdenum antimony scandium method and mo⁃lecular biology identification.Semi⁃solid plant inoculation test was conducted to explore bacterial strain ability to dis⁃solve inorganic phosphorus and promote the growth of ryegrass.Preliminary identification was carried out.【Result】The dissolved inorganic phosphorus contents of eight bacterial strains were between 329.39 and 479.09μg/mL after culturing in PKO liquid medium for 10 d,of the eight strains,strain MPD4 had the highest amount of dissolved phos⁃phorus.All eight bacterial strains belonged to the genus Pseudomonas.【Conclusion】The plant inoculation test showed that compared with the blank control,the eight strains could effectively promote the growth and development of ryegrass.This study provides an excellent strain resource for the further exploration of the growth⁃promoting effect of Pseudomonas phosphate⁃solubilizing bacteria and the production of microbial fertilizers.