一株植物乳杆菌菌体富硒条件的优化

Optimization of selenium enrichment condition for a Lactobacillus plantarum

本研究旨在探究从四川传统发酵米制品中分离出1株植物乳杆菌(Lactiplantibacillus plantarum) BM-5的菌体富硒功能.通过正交实验确定最佳富硒条件为pH 7.0、添加亚硒酸钠使硒添加浓度为30μg/mL、硒添加时间为12 h、接种量为5%.在这些条件下BM-5菌的菌体生物量1.12 g/L,菌体硒含量可达7362.60μg/g.通过扫描电镜观察和能谱分析证实BM-5能富集还原硒并可将部分单质硒由菌体内运输到菌体表面形成纳米硒颗粒,纳米硒颗粒的平均直径为167.16 nm.该研究表明BM-5在较低硒添加浓度时,可被制备成结合富硒和益生的多功能菌株,具备应用于生产安全的人体硒补充剂的潜力.

The purpose of this study was to investigate the selenium enrichment function of Lactiplantibacil-lus plantarum BM-5 isolated from traditional fermented rice products in Sichuan Province.The optimal selenium enrichment conditions were determined by orthogonal experiment as pH 7.0,selenium concentration 30µg/mL,selenium addition time 12 h and inoculation amount 5%with sodium selenite.Under these conditions,the biomass of BM-5 was 1.12 g/L,and the selenium content of BM-5 reached 7362.60μg/g.Scanning electron microscopy and energy spectrum analysis showed that BM-5 can enrich and reduce selenium and transport some elemental selenium from the bacteria to the surface of the bacteria to form nano-selenium particles,the average diameter of nano-selenium particles was 167.16 nm.This study showed that BM-5 could be prepared into a strain combining selenium enrichment and prebiotics at a low selenium concentration,which had the potential to be used in the production of safe selenium supplements for human.