Effect of moxibustion on colonic low-grade inflammatory response in rats with diarrhea-predominant irritable bowel syndrome based on mast cell degranulation

Objective To observe the effects of moxibustion on colonic mast cell degranulation and inflammatory factor expression in rats with diarrhea-predominant irritable bowel syndrome(IBS-D),and explore the potential mechanism of moxibustion in treating IBS-D.Methods In forty-five rat pups born from 5 healthy SPF-grade pregnant SD rats,8 rats were randomly selected as normal group,and the remaining 37 rats were intervened with maternal separation,acetic acid enema,and chronic restraint stress to establish the IBS-D model.The successfully modeled 32 rats were then randomly assigned to a model group,a ketotifen group,a moxibustion group,and a moxibustion-medication group,with 8 rats in each group.The rats in the ketotifen group were intervened with intragastric administration of ketotifen solution(10 mL/kg).