Effect of Dapagliflozin on cell proliferation, apoptosis and inflammation of podocytes induced by high glucose via regulating p38 MAPK pathway

Objective To investigate the effect of Dapagliflozin on high glucose-induced podocyte proliferation and apoptosis through p38 mitogen-activated protein kinase(p38 MAPK)pathway.Methods Human glomerular podocytes(HGPC)were divided into control(Con)group,low/medium/high D-glucose(Glu 10,Glu 20,Glu 30)group,high glucose(HG)group,low/medium/high concentration Dapagliflozin(HG+Dap 12.5,HG+Dap 25,HG+Dap 50)group,Dapagliflozin(HG+Dap)group,inhibitor(HG+SB 203580)group,Dapagliflozin+inhibitor(HG+Dap+SB 203580)group and Dapagliflozin+activator(HG+Dap+C16-PAF)group.After 24 hours of intervention,the cell viability,proliferation rate,apoptosis rate and levels of related factors were tested.Results Compared with Con group,IL-1β,TNF-α,apoptosis rate,Caspase-3 mRNA and protein expression,p53,P-p38 MAPK protein expression were increased(P<0.05),while cell proliferation rate,Cyclin D1 mRNA and protein expression were decreased in HG group(P<0.05).Compared with HG group,the proliferation rate,Cyclin D1 mRNA and protein expression were increased(P<0.05),while IL-1β,TNF-α,apoptosis rate,Caspase-3 mRNA and protein expression,p53,P-p38 MAPK protein expression were decreased in the HG+Dap and HG+SB 203580 groups(P<0.05).Compared with HG+Dap group,cell proliferationn rate.