Effect of miR-130a targeting phosphase and tensin homology deleted on chromosome ten/ phosphoinositol 3 kinase/protein kinase B axis on apoptosis of renal tissue cells in rats with diabetic kidney disease

Objective To investigate the effect of miR-130a targeting phosphase and tensin homology deleted on chromosome ten(PTEN)/phosphoinositide 3 kinase(PI3K)/protein kinase B(AKT)pathway on renal tissue cell apoptosis in diabetic kidney disease(DKD)rats.Methods The DKD rat model was constructed by feeding high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin(STZ).72 rats were divided into normal control group(NC),DKD model group(DKD),miR-13Oa agonist negative control group(NC agomir),and miR-130a agonist group(miR-130a agomir),miR-130a agomir+PTEN overexpression negative control group(miR-13a agomir+pcDNA),and miR-130a agomir+PCDNA-PTEN over-expression group(miR-130a Agomir+PCDNA-PTEN),with 12 rats in each group.Urinary microalbumin kit was used to detect 24 h urine albumin(UAlb).Fasting blood glucose(FBG),serum creatinine(Scr)and blood urea nitrogen(BUN)were detected by automatic biochemical analyzer.Pathological changes of renal tissue were detected by HE staining.The levels of serum IL-6 and TNF-αwere detected by ELISA.The apoptosis of renal tissue was detected by TUNEL staining.The expression of miR-130a was detected by qRT-PCR,and the expression of B-cell lymphoma-2-associated X protein(Bax).